FlyBase Allele Report: Dmel\Yp1[ts1]

General Information

Symbol

Dmel\Yp1^ts1

Species

D. melanogaster

Name

FlyBase ID

FBal0018810

Feature type

allele

Associated gene

Dmel\Yp1

Associated Insertion(s)

Carried in Construct

Also Known As

fs(1)1163

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Ile at residue 92 replaced by Asn.

(Butterworth et al., 1992)

Nucleotide substitution: C?T. Nucleotide substitution: T?A. Amino acid replacement: I92N. Mutations are in the second exon.

(Saunders and Bownes, 1986)

ile92->asn92

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:10,054,268..10,054,268 [+]

Nucleotide change:

T10054268A

Reported nucleotide change:

T?A

Amino acid change:

I92N | Yp1-PA; I92N | Yp1-PB

Reported amino acid change:

I92N

(Saunders and Bownes, 1986)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Minoo and Postlethwait, 1985, Postlethwait and Shirk, 1981)

female sterile | dominant | heat sensitive

(Butterworth et al., 1991)

female sterile | recessive

(Butterworth et al., 1991)

female sterile soma-dependent | dominant | heat sensitive

(Wieschaus et al., 1981)

Phenotype Manifest In

adult fat body | heat sensitive

(Butterworth et al., 1991)

egg | heat sensitive

(Wieschaus et al., 1981)

Detailed Description

Statement

Reference

Osmium zinc iodide staining in Golgi of ovarian follicles is wild type, but no yolk spheres label.

(Giorgi et al., 1993)

Yolk protein aggregates found in space between plasmalemma and vitelline membranes and embedded within the vitelline membrane itself. This interferes with correct assembly of the egg shell membranes, causing sterility.

(Butterworth et al., 1992)

Heterozygous females are fertile and secrete Yp1 protein at 18^oC, but are sterile and do not secrete Yp1 protein at 29^oC. Homozygous females are sterile and fail to secrete Yp1 protein at both temperatures. Unusual electron-dense material is found in the subbasement membrane space of many fat body cells in heterozygotes at 29^oC. This subbasement membrane material (SBMM) is often located near the plasma membrane, and consists of two types of material, a material which sometimes appears fibrous with a crystalline, lattice-shaped morphology, and an amorphous material. The SBMM contains yolk. Homozygous females reared at either 20 or 29^oC have smaller fat body cells than normal, and SBMM is present. Yp1^ts1/Df(1)C52 females have an abnormal fat body. SBMM is present in females reared at 29^oC.

(Butterworth et al., 1991)

Homozygous follicle cells differ from wild-type by having larger secretory vesicles, forming clumps of an electron-dense secretory component within the developing vitelline membrane (this component disperses after secretion in the wild-type), accumulating more tubules in the cortical ooplasm of vitellogenic oocytes, and possessing altered yolk spheres. Homozygous ovaries transplanted into wild-type hosts show no improvement in the secretory granule phenotype and slight improvement of the vitelline membrane clump phenotype, but show amelioration of the oocyte tubule and yolk sphere phenotypes.

(Giorgi and Postlethwait, 1985)

Homozygous females are sterile.

(Postlethwait and Shirk, 1981)

Heterozygous females lay normal eggs at 18^oC whereas all eggs produced at 29^oC are collapsed.

(Wieschaus et al., 1981)

female-sterile Homozygous female sterile at 18^oC and homo- and heterozygous female sterile at 29^oC, producing flaccid eggs and reduced amounts of yolk protein at the high temperature. (Bownes and Hodson, 1980). Yp1^ts1 is female sterile over Df(1)C52, a deficiency for the Yp1 locus (Postlethwait and Shirk, 1981).

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

4568

Yp1^ts1 w¹ cv¹ v¹/C(1)DX, y¹ f¹

Notes on Origin

Discoverer

Comments

Germ line clonal analysis shows that Yp1 function is required in the soma.

(Wieschaus et al., 1981)

Yp1 gene product deficiency in haemolymph and eggs due to reduced rate of secretion.

(Minoo and Postlethwait, 1985)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Yp1¹¹⁶³

(Minoo and Postlethwait, 1985)

Yp1^ts1

(Shima et al., 2025, Cook et al., 2009.2.28, Christensen et al., 2008.6.11, Christensen et al., 2008.12.28)

Yp1^ts

(Andersen and Horne-Badovinac, 2016)

fs(1)1163

(Giorgi et al., 1993, Butterworth et al., 1992, Bownes et al., 1991, Butterworth et al., 1991, Saunders and Bownes, 1986, Giorgi and Postlethwait, 1985, Minoo and Postlethwait, 1985, Postlethwait and Shirk, 1981, Wieschaus et al., 1981, Bownes and Hodson, 1980)

Name Synonyms

Secondary FlyBase IDs

References (16)

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