Isolation and characterization of Df(1)BSC712
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC712 was isolated as a FLP recombinase-induced recombination event involving PBac{RB}Aats-lyse04554 and P{XP}CG2974d03231. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of w1118 PBac{RB}Aats-lyse04554/w1118 P{XP}CG2974d03231; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.RB5}BSC712 from the segment of PBac{RB}Aats-lyse04554 to the left of its FRT site and the segment of P{XP}CG2974d03231 to the right of its FRT site. Exelixis, Inc. determined the insertion site of P{XP}CG2974d03231 to be Release 3 genomic coordinate 9826334 on the X chromosome. This corresponds to 9B1 on the Release 3 and Release 5 genome maps. The predicted position of PBac{RB}Aats-lyse04554 on the Release 5 map is 8F1. Consequently, the cytological breakpoints of Df(3L)BSC712 are predicted to be 8F1;9B1. It failed to complement Yp1ts1.