FlyBase Allele Report: Dmel\Antp[hs.PHTA]

General Information

Symbol

Dmel\Antp^hs.PHTA

Species

D. melanogaster

Name

FlyBase ID

FBal0028955

Feature type

allele

Associated gene

Dmel\Antp

Associated Insertion(s)

Carried in Construct

P{hsp70-Antp}

Also Known As

HSAntp

Key Links

Transgenic product class

wild_type

(Gibson et al., 1990)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Gibson et al., 1990)

Mutagen

in vitro construct

(Brizuela and Kennison, 1997, Haerry and Gehring, 1996, Chan and Mann, 1993, Pelaz et al., 1993, Zhao et al., 1993, Gonzalez-Reyes et al., 1992, Heuer and Kaufman, 1992, Roder et al., 1992, Wagner-Bernholz et al., 1991, Gibson et al., 1990, Gonzalez-Reyes et al., 1990, Schneuwly et al., 1987)

Progenitor genotype

Carried in construct

P{hsp70-Antp}

Cytology

Description

Antp gene expression is driven by an Hsp70 promoter.

(Gibson et al., 1990)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Antp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal segment 1

(Sanchez-Herrero et al., 1994)

adult prothoracic segment

(Malicki et al., 1990)

antenna

(Brizuela and Kennison, 1997, Zhao et al., 1993, Malicki et al., 1990)

antenna | heat sensitive

(Vazquez et al., 1999, Schneuwly et al., 1987)

arista

(Kuzin et al., 1997)

embryonic head

(Malicki et al., 1990)

embryonic head | heat sensitive

(Heuer and Kaufman, 1992)

embryonic head segment | dorsal

(Gibson and Gehring, 1988)

embryonic head segment | dorsal | heat sensitive

(Bello et al., 1998)

embryonic mesothoracic segment | heat sensitive

(Heuer and Kaufman, 1992)

embryonic metathoracic segment | heat sensitive

(Heuer and Kaufman, 1992)

embryonic prothoracic segment | heat sensitive

(Heuer and Kaufman, 1992)

external sensory organ | heat sensitive

(Heuer and Kaufman, 1992)

head

(Sanchez-Herrero et al., 1994)

head | dorsal | heat sensitive

(Schneuwly et al., 1987)

head | heat sensitive

(Gonzalez-Reyes et al., 1990)

larval head segment | heat sensitive

(Li and McGinnis, 1999)

leg

(Gibson and Gehring, 1988)

mesothoracic dorsal triscolopidial chordotonal organ dch3 | heat sensitive

(Heuer and Kaufman, 1992)

metathoracic dorsal triscolopidial chordotonal organ dch3 | heat sensitive

(Heuer and Kaufman, 1992)

ommatidium

(Bhojwani et al., 1997)

prothoracic segment | heat sensitive

(Gonzalez-Reyes et al., 1990)

prothoracic ventral denticle belt | embryonic stage

(Gibson and Gehring, 1988)

prothoracic ventral denticle belt | embryonic stage | heat sensitive

(Bello et al., 1998)

prothoracic ventral denticle belt | heat sensitive

(Li and McGinnis, 1999)

second segment of antenna

(Kuzin et al., 1997)

second segment of antenna | heat sensitive

(Wagner-Bernholz et al., 1991)

third segment of antenna

(Kuzin et al., 1997)

thorax

(Sanchez-Herrero et al., 1994)

ventral denticle belt

(Chan and Mann, 1993)

ventral denticle belt | embryonic stage

(Malicki et al., 1990)

Detailed Description

Statement

Reference

The ectopic expression of Antp^hs.PHTA (driven by heat shock), results in a denticle transformation of the head and T1 segments to T2-like morphologies. This phenotype resembles that of Ubx^{Ia.Scer\UAS.T:Hsim\VP16} driven by Scer\GAL4^arm.PS.

(Li and McGinnis, 1999)

Expression of Antp^hs.PHTA during the larval stages results in antenna to leg transformations. This phenotype is not affected if the animals are also carrying osa¹ or osa².

(Vazquez et al., 1999)

30 minute heat shock at 6.5 hours of embryogenesis causes failure of head involution in early embryos, defects on the dorsal surface of the head and disruptions of the prothoracic denticle belts.

(Bello et al., 1998)

Heat induced expression in tsh mutants background increases the frequency of the reduced-eye phenotype (ommatidia are replaced by micro- and macrochaetae).

(Bhojwani et al., 1997)

Produces antenna to leg transformations when expressed using heat shock. The penetrance of these transformations is not suppressed by mor¹ or mor².

(Brizuela and Kennison, 1997)

Heat induced expression does not influence formation of different tarsal structures but often causes thickening of the arista and enlargement of the second and third antennal segments.

(Kuzin et al., 1997)

Heat induced coexpression with abd-A^hs.PS between 3 and 5 hours of development causes transformation of thoracic segments to A2-A6 identity. Transformation of the first abdominal segment to a more posterior abdominal segment, A2-A6 identity. Appearance of two segments on the head as shown be the denticle belts. Posterior structures are not transformed. Coexpression with Ubx^hs.PG shows similar transformation to expression of Ubx^hs.PG alone.

(Sanchez-Herrero et al., 1994)

tsh^hs.PdZ increases the expressivity of the homeotic transformations caused by Antp^hs.PHTA or Ubx^hs.PG.

(de Zulueta et al., 1994)

Embryos exhibit transformation of parasegment 4.

(Chan and Mann, 1993)

High levels of Antp repress Scr in the posterior compartments of the thorax and abdomen.

(Pelaz et al., 1993)

Transformation of antennae toward T2 leg.

(Zhao et al., 1993)

Double antibody staining with F2 and P12α-85E revealed that T2 or T3 dch3 were occasionally in an abnormal position but the most severe defects were seen in T1 es organs. Heat induction causes transformation of head and thoracic segments towards abdominal segment 1 identity. dch3 organs of thoracic segments 2 and 3 are not transformed to an lch5 identity but to lch3. There is an abdominal segment 1 pattern of es organs in thoracic segments 1, 2 and 3.

(Heuer and Kaufman, 1992)

Heat induction causes transformation of the second antennal segment to the corresponding leg segment, trochanter.

(Wagner-Bernholz et al., 1991)

Without heat induction components of the head and cephalopharyngeal skeleton fail to fuse and occupy the fixed and characteristic positions. Heat induction enhances this mutant phenotype.

(Gonzalez-Reyes and Morata, 1990)

Heat induction causes transformation of head segments and thoracic segment 1 to thoracic segment 2 identity.

(Gonzalez-Reyes et al., 1990)

Heat induced expression during embryogenesis causes disruption of head involution. Thoracic denticles appear on the lateral and dorsolateral aspects of the head and dorsal thoracic hairs appear on the dorsal aspect of the head. Expression during third larval instar causes a transformation of first thoracic segment toward second thoracic identity. Also transformation of adult antenna to second thoracic leg.

(Malicki et al., 1990)

Head involution is prevented and T1 is partially transformed towards T2 in heat shocked larvae. Heat shocked larvae also mutant for exd show a transformation of T1 towards an intermediate T1/T2 morphology.

(Peifer and Wieschaus, 1990)

Ectopic expression in embryonic stages causes failure of head involution in early embryos, defects on the dorsal surface of the head and disruptions of the prothoracic denticle belts. Late embryonic induction causes larval lethality. Induction later in development causes leg transformation in the viable adults, distal to proximal transformations of antenna towards leg.

(Gibson and Gehring, 1988)

Heat shock during the third larval stage causes transformation of antennae to legs and dorsal surfaces of the head transformed to mesothoracic scutem structures. No suppression of the formation of the thoracic segment specific sensory organs (Keilin's organ and ventral pits).

(Schneuwly et al., 1987)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Antp^hs.PHTA, obk¹ has visible phenotype

(Dworkin et al., 2001)

Antp^hs.PHTA, ss^aSc has abnormal size | adult stage phenotype

(Kuzin et al., 1997)

Antp^hs.PHTA, ss^aSc has visible phenotype

(Kuzin et al., 1997)

Phenotype Manifest In

Suppressor of

Statement

Reference

Antp^hs.PHTA is a suppressor of phenotype of spen^poc361

(Wiellette et al., 1999)

Other

Statement

Reference

Antp^hs.PHTA, obk¹ has leg | ectopic phenotype

(Dworkin et al., 2001)

Antp^hs.PHTA, obk¹ has antenna phenotype

(Dworkin et al., 2001)

Antp^hs.PHTA, ss^aSc has tarsal segment phenotype

(Kuzin et al., 1997)

Antp^hs.PHTA, ss^aSc has third segment of antenna phenotype

(Kuzin et al., 1997)

Antp^hs.PHTA, ss^aSc has second segment of antenna phenotype

(Kuzin et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

obk¹, Antp^hs.PHTA produces bi- or polyramous appendages with both antenna and leg combinations after heat shocks of 40 min duration at either 56 or 60 hours after hatch.

(Dworkin et al., 2001)

Induction of expression in ss^aSc flies causes severe tarsal defects, tarsal structures resemble mini-tibia. Antennal structures look unusual due to overgrowth of the second and third antennal segments and proximal structures of the homeo tarsus.

(Kuzin et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Schneuwly.

(Zhao et al., 1993, Roder et al., 1992, Wagner-Bernholz et al., 1991, Gibson et al., 1990)

Gonzalez-Reyes.

(Pelaz et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Antp^H4

(Wagner-Bernholz et al., 1991)

Antp^hs.PHTA

Name Synonyms

Secondary FlyBase IDs

FBal0033436

References (33)

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