FlyBase Allele Report: Dmel\N[ECN.hs]

General Information

Symbol

Dmel\N^ECN.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0029994

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{N^hs.ECN}

Key Links

Transgenic product class

dominant_negative_variant

(Rebay et al., 1993)

inframe_deletion

(Rebay et al., 1993)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Rebay et al., 1993)

inframe_deletion

(Rebay et al., 1993)

Mutagen

in vitro construct

(Rebay et al., 1993)

Progenitor genotype

Carried in construct

P{N^hs.ECN}

Cytology

Description

An Hsp70 promoter drives expression of a N minigene (contains genomic and cDNA sequence) that carries a deletion of the entire intracellular domain (the extracellular and transmembrane domains are present). The encoded protein acts as a dominant negative form of N.

(Rebay et al., 1993)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | heat sensitive

(Escudero et al., 2003)

Phenotype Manifest In

microchaeta | heat sensitive

(Escudero et al., 2003)

Detailed Description

Statement

Reference

Expression of N^ECN.hs using heat shock results in loss of microchaetae on a small region of the notum.

(Escudero et al., 2003)

Heat shock causes a "dominant loss of function" type phenotype of aberrant wing vein pattern, wing nicking, severe rough eye with scarring and missing thoracic microchaetae.

(Rebay et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

N^ECN.hs has visible | heat sensitive phenotype, non-suppressible by ed^ΔECD.UAS/Scer\GAL4^sca-537.4

(Escudero et al., 2003)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

N^ECN.hs has microchaeta | heat sensitive phenotype, non-suppressible by ed^ΔECD.UAS/Scer\GAL4^sca-537.4

(Escudero et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The loss of microchaetae seen in animals expressing N^ECN.hs using heat shock is not rescued by expression of ed^{ΔECD.Scer\UAS} under the control of Scer\GAL4^sca-537.4.

(Escudero et al., 2003)

N^ECN.hs mutants have no discernible inhibitory effect on sensory organ precursor cells in aph-1^D35 wing discs.

(Hu and Fortini, 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

N^ECN.hs

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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