FlyBase Allele Report: Dmel\ninaE[ΔAsn20]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\ninaE^ΔAsn20

General Information

Symbol

Dmel\ninaE^ΔAsn20

Species

D. melanogaster

Name

FlyBase ID

FBal0032281

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{ninaE^ΔAsn20}

Also Known As

ΔAsn₂₀

Key Links

Transgenic product class

missense_variant

(O'Tousa, 1992)

polypeptide_post_translational_processing_variant

(O'Tousa, 1992)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(O'Tousa, 1992)

polypeptide_post_translational_processing_variant

(O'Tousa, 1992)

Mutagen

in vitro construct

(Katanosaka et al., 1998, Brown et al., 1994, O'Tousa, 1992)

Progenitor genotype

Carried in construct

P{ninaE^ΔAsn20}

Cytology

Description

Amino acid replacement: N20I.

(Kurada and O'Tousa, 1995)

Amino acid replacement: N20I. Nucleotide substitution: A?T. N-linked glycosylation site of opsin is mutated.

(Brown et al., 1994)

Construct: Amino acid replacement: N20I. N-linked glycosylation site near amino terminus is removed.

(O'Tousa, 1992)

Allele components

Component

Use(s)

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology

(Webel et al., 2000)

Phenotype Manifest In

photoreceptor cell & Golgi apparatus

(Webel et al., 2000)

photoreceptor cell & rough endoplasmic reticulum

(Webel et al., 2000)

photoreceptor cell R1

(Brown et al., 1994)

photoreceptor cell R2

(Brown et al., 1994)

photoreceptor cell R3

(Brown et al., 1994)

photoreceptor cell R4

(Brown et al., 1994)

photoreceptor cell R5

(Brown et al., 1994)

photoreceptor cell R6

(Brown et al., 1994)

(Webel et al., 2000, O'Tousa, 1992)

(Webel et al., 2000, Brown et al., 1994)

(Webel et al., 2000, Brown et al., 1994)

(Webel et al., 2000, Brown et al., 1994)

(Webel et al., 2000, Brown et al., 1994)

(Webel et al., 2000, Brown et al., 1994)

(Webel et al., 2000, Brown et al., 1994)

Detailed Description

Statement

Reference

The rhabdomeres are severely reduced in size in 1 day old flies carrying ninaE^ΔAsn20 in a ninaE¹⁷ background. Photoreceptors show accumulations of rough ER cisternae and dilated Golgi. Electroretinograms of 3-5 day old flies show a reduced amplitude of light response and lack a prolonged depolarising afterpotential. Severe retinal degeneration is seen in 5 week old flies; the R1-R6 rhabdomeres are completely lost. Flies carrying both ninaE^ΔAsn20 and ninaE^{hs.T:Btau\1D4} in a ninaE¹⁷ background show an accumulation of ER cisternae in the photoreceptors (as is seen in the photoreceptors of flies carrying only ninaE^ΔAsn20 in a ninaE¹⁷ background). 5 week old flies carrying both ninaE^ΔAsn20 and ninaE^{hs.T:Btau\1D4} in a ninaE¹⁷ background show retinal defects including reduced rhabdomere size, internalized rhabdomeres, membrane accumulations, and progressive, late onset, retinal degeneration.

(Webel et al., 2000)

Microspectrophotometry (MSP) reveals minimal photopigment within a week after eclosion. Electroretinography (ERG) demonstrates that receptor cell 1-6 function is decreased 100-fold at eclosion and diminishes until only receptor cell 7 and 8 function at 11 days The PDA is small and starts to diminish at 7 days, the on off transients show the same pattern and sensitivity decreases with age. Morphometry shows the rhabdomeres are small and may decrease in size with age.

(Brown et al., 1994)

Expressed in ninaE¹⁷ flies. Levels of rhodopsin are similar to that of ninaE³ and ninaE⁵. ERG responses show residual rhodopsin is capable of initiating phototransduction, but there is no PDA. Rhabdomere organisation is aberrant and undergoes age-dependent deterioration.

(O'Tousa, 1992)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

ninaE^ΔAsn20 is a suppressor of phenotype of norpA^P24

(Kurada and O'Tousa, 1995)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

64823

P{ninaE^ΔAsn20}2/CyO; ninaE¹⁷ e^s ry⁵⁰⁶

64125

P{ninaE^ΔAsn20}1, w^*; ry⁵⁰⁶ ninaE¹⁷ e^s

64126

P{ninaE^ΔAsn20}1, w^*; ninaA³; ninaE¹⁷ e^s

Notes on Origin

Discoverer

Comments

Comments

Mutation interferes with either synthesis, deployment or maintenance of opsin.

(Brown et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

N20I

(Bentrop, 1998)

Rh1^N20I

(Webel et al., 2000)

ninaE^ΔAsn20

ΔAsn₂₀

(Shim et al., 1997, Brown et al., 1994, O'Tousa, 1992)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections