FlyBase Allele Report: Dmel\pnr[VX1]

General Information

Symbol

Dmel\pnr^VX1

Species

D. melanogaster

Name

FlyBase ID

FBal0032465

Feature type

allele

Associated gene

Dmel\pnr

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

gain of function allele

antimorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Heitzler et al., 1996, Ramain et al., 1993)

gain of function allele

(Calleja et al., 1996, Heitzler et al., 1996)

Mutagen

X ray

(Heitzler et al., 1996, Ramain et al., 1993)

Progenitor genotype

Cytology

Description

14 nucleotide deletion, between nucleotides 1715 and 1728, causing a frameshift stop truncating the protein to 514 amino acids, and removing the putative α-helices.

(Ramain et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:16,039,910..16,039,923 [+]

Comment:

14 nucleotide deletion causing a frameshift stop truncating the protein to 514 amino acids

(Ramain et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Heitzler et al., 1996, Ramain et al., 1993)

visible | dominant

(Heitzler et al., 1996, Ramain et al., 1993)

Phenotype Manifest In

anterior dorsocentral bristle

(Ramain et al., 1993)

dorsal longitudinal indirect flight muscle cell (with pnr^V1)

(Ghazi et al., 2003)

dorsocentral bristle

(Vanolst et al., 2005, Calleja et al., 1996, Heitzler et al., 1996)

posterior dorsocentral bristle

(Ramain et al., 1993)

scutellar bristle

(Calleja et al., 1996)

Detailed Description

Statement

Reference

pnr^VX1 heterozygotes exhibit a loss of dorsocentral bristles compared to wild-type (1.86 bristles per heminotum compared to 2.0 in wild-type controls).

(Vanolst et al., 2005)

pnr^V1/pnr^VX1 mutants show abnormal muscle attachments. The dorsal medial muscle (DLM)s appear as a single fibre that resembles the lateral oblique dorsal muscle (DVM III) in its orientation.

(Ghazi et al., 2003)

In pnr^V1/pnr^VX1 mutants the wg-dependent dorsocentral bristles and scutellar bristles are missing.

(Calleja et al., 1996)

Lethality occurs at larval or early pupal stages. Heterozygotes occasionally lack one dorsocentral bristle. Heterozygotes with pnr^V1 are viable.

(Heitzler et al., 1996)

Heterozygotes are missing one of the two dorsocentral bristles. Homozygous clones of cells are missing one or both bristles. Dominant phenotype enhanced in viable transallelic combinations with pnr^VX1. Homozygotes die as young larvae with no obvious mutant phenotype.

(Ramain et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

pnr^VX1 has dorsocentral bristle phenotype, enhanceable by Iswi²

(Vanolst et al., 2005)

pnr^VX1 has dorsocentral bristle phenotype, enhanceable by tou²

(Vanolst et al., 2005)

Suppressor of

Statement

Reference

pnr^VX1 is a suppressor of dorsocentral bristle phenotype of tou²

(Vanolst et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

tou²/+ ; pnr^VX1/+ flies exhibit on average 1.57 dorsocentral bristles per heminotum, compared to 1.86 bristles per heminotum in pnr^VX1 heterozygotes, 1.4 bristles per heminotum in tou²/+ and 2.0 dorsocentral bristles per heminotum in wild-type. Iswi²/+ ; pnr^VX1/+ flies display on average 1.61 dorsocentral bristles per heminotum, so aggravating the pnr^D1/+ single mutant phenotype (1.86 bristles/heminotum).

(Vanolst et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Class B allele.

(Ramain et al., 1993)

Class 3 pnr allele: dominant allele causing loss of bristles.

(Heitzler et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

pnr^VX1/4

(Tomoyasu et al., 2000)

pnr^VX1

(Fromental-Ramain et al., 2010)

pnr^vx1

(Simpson, 1997)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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