FlyBase Allele Report: Dmel\Adcy1[rut-1084]

General Information

Symbol

Dmel\Adcy1^rut-1084

Species

D. melanogaster

Name

FlyBase ID

FBal0032623

Feature type

allele

Associated gene

Dmel\Adcy1

Associated Insertion(s)

P{lArB}Adcy1^rut-1084

Carried in Construct

Also Known As

rut¹⁰⁸⁴

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

P-element activity

(Levin et al., 1992)

Delta2-3 transposase

(Han et al., 1996, Han et al., 1992)

Progenitor genotype

P{lArB}A4.1M2

(Han et al., 1996)

Associated Insertion(s)

P{lArB}Adcy1^rut-1084

Cytology

Description

P{lArB} insertion within 200bp upstream of the presumed transcription start site.

(Han et al., 1996)

Allele components

Component

Use(s)

Inserted element

P{lArB}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning | semidominant

(Han et al., 1992)

abnormal memory | adult stage

(Ishimoto et al., 2013)

abnormal neurophysiology

(Renger et al., 2000)

Phenotype Manifest In

neuromuscular junction

(Renger et al., 2000)

Detailed Description

Statement

Reference

Mutant males lack 30-minute courtship memory after 1 hour of conditioning in a courtship conditioning assay. This defect is significantly suppressed if the males are fed 0.1mM 20-hydroxy-ecdysone for 10 minutes immediately prior to conditioning.

(Ishimoto et al., 2013)

The frequency of spontaneous release (miniature excitatory junctional currents) at the neuromuscular junction is reduced compared to wild type.

(Renger et al., 2000)

Severe defects in initial learning and in 60 minute memory retention, measured in negatively reinforced olfactory discrimination paradigm.

(Han et al., 1992)

In an odour avoidance paradigm exhibits significantly reduced learning.

(Levin et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Adcy1^rut-1084 has abnormal memory | adult stage phenotype, suppressible | RU486 conditional | drug conditional by Scer\GAL4^{Mef2.247.Switch}/DopEcR^UAS.cIb

(Ishimoto et al., 2013)

NOT suppressed by

Statement

Reference

Adcy1^rut-1084 has abnormal memory | adult stage phenotype, non-suppressible | RU486 conditional | drug conditional by Scer\GAL4^{Mef2.247.Switch}/DopEcR^UAS.cIb

(Ishimoto et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of DopEcR^Scer\UAS.cIb under the control of Scer\GAL4^{Mef2.247.Switch} in adult mushroom body neurons (using RU486 to limit the timing of expression) does not restore courtship memory in a courtship conditioning assay in rut¹⁰⁸⁴ males. However, if these flies are also fed 0.01mM 20-hydroxy-ecdysone (a concentration that does not rescue courtship memory in rut¹⁰⁸⁴ single mutants), significant rescue of courtship memory is seen.

(Ishimoto et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Adcy1^rut-1

(Levin et al., 1992)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Adcy1^rut-1084

(FlyBoard ad hoc nomenclature committee, 2024.7.14)

rut¹⁰⁸⁴

(Ishimoto et al., 2013, Lee and Wu, 2010)

rut^MB1084

(Han et al., 1992)

Name Synonyms

Secondary FlyBase IDs

FBal0032626

References (7)

Report Sections

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