Marker expression analysis shows that rhoPΔ5 homozygous eyes (generated using 'ey-FLP' + Scer\FRT) the proportion of pale-ommatidia is reduced and hybrid ommatidia are present with a yellow-type R8 and a pale-type R7. In eyes that are a mosaic of rhoPΔ5 homozygous, heterozygous and wild-type clones, there is a significant decrease in the proportion of pale-type R8 cells in pale ommatidia in ommatidia where both R8 are R7 are not rhoPΔ5 homozygous. This proportion is not significantly different in ommatidia with a rhoPΔ5 homozygous R7 and wild-type of heterozygous R8, but is decreased significantly more in ommatidia with a rhoPΔ5 homozygous R8, irrespective of the genotype of R7.
The tracheal system of rhoPΔ5 embryos show branch interruptions and branches with cells only connected by cytoplasmic extensions. The tracheal tubes of stage 16 rhoPΔ5 embryos show a thinner accumulation of cortical actin compared to controls.
Ommatidia in homozygous clones sometimes have missing photoreceptor cells.
Homozygous clones in the adult abdomen do not show any consistent alterations of normal polarity.
The number of chordotonal organs in the lateral cluster is reduced from 5 to 3 in stage 16 homozygous embryos and there is a complete lack of oenocytes. Oenocyte precursor whorls are missing in stage 11 embryos.
Homozygous embryos show a variable tracheal phenotype consisting mostly of defects in migration along the anteroposterior axis. The dorsal trunk and visceral branches are missing or incomplete. Some tracheal cells remain in the ectoderm at stage 12 in contrast to wild type.
Embryos show normal guts.
No effect on the faf eye phenotype.
Germline clone experiments show that rho has no function in oogenesis.
Defects in embryonic head skeleton, and characteristic fusions of denticle belts.