FlyBase Allele Report: Dmel\N[Hsp70Bb.PL]

General Information

Symbol

Dmel\N^Hsp70Bb.PL

Species

D. melanogaster

Name

heat shock construct of Lieber

FlyBase ID

FBal0033870

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{hsN}

Also Known As

hsN, hs-N⁺, hs-N

Key Links

Transgenic product class

wild_type

(Lieber et al., 1992)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Lieber et al., 1992)

Mutagen

in vitro construct

(Lieber et al., 1993)

Progenitor genotype

Carried in construct

P{hsN}

Cytology

Description

Hsp70Bb heatshock promoter sequences drive fused wildtype N genomic and cDNA sequences with all but the last three introns deleted. A genomic N polyA tail has also been added.

(Lieber et al., 1992)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70Bb

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory, with Scer\GAL4^hs.PB

(Zhang et al., 2013)

abnormal memory | heat sensitive

(Matsuno et al., 2009, Ge et al., 2004)

Phenotype Manifest In

S2 cell-line

(Ahimou et al., 2004)

Detailed Description

Statement

Reference

N^Hsp70Bb.PL mutant flies exhibit significantly enhanced 24 hour long-term memory formation at both 30 and 37[o]C.

(Zhang et al., 2013)

Heat-shocked N^Hsp70Bb.PL flies show a greater than 4-fold increase in 1-day memory after single-cycle training.

(Matsuno et al., 2009)

The surface topology of cells expressing N^Hsp70Bb.PL is different from that of control cells.

(Ahimou et al., 2004)

Adult flies expressing N^Hsp70Bb.PL (using a 37^oC heat shock) show normal reactivity to electric shock and normal olfactory acuity (to methylcyclohexanol and octanol). Flies in which N^Hsp70Bb.PL is expressed using a 30 minute heat shock show normal learning scores in a Pavlovian learning and memory assay, when trained and tested 3 hours after the heat shock. One day memory is also unaffected in these flies when they are subjected to the normal spaced training procedure (10 training cycles), however, flies that have only one or two spaced training sessions show enhanced one day memory compared to control flies.

(Ge et al., 2004)

Has no effect on the development of otherwise wild type embryos.

(Lopez-Schier and St. Johnston, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

N^Hsp70Bb.PL has abnormal memory | heat sensitive phenotype, suppressible by klg^ruslan

(Matsuno et al., 2009)

N^Hsp70Bb.PL has abnormal memory | heat sensitive phenotype, suppressible by klg^E226/klg^GS10439

(Matsuno et al., 2009)

NOT Suppressor of

Statement

Reference

N^Hsp70Bb.PL is a non-suppressor of lethal | neurogenic phenotype | recessive | germline clone phenotype of Nct^agro

(Lopez-Schier and St. Johnston, 2002)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The enhancement of long-term memory by N^Hsp70Bb.PL over-expression is suppressed in klg^ruslan or klg^GS10439/klg^E226 flies.

(Matsuno et al., 2009)

Cells co-expressing O-fut1^dsRNA.cAa and N^Hsp70Bb.PL show a detachment force from Dl^hs.PL-expressing cells of almost zero.

Cells co-expressing O-fut1^dsRNA.cAa and N^Hsp70Bb.PL do not form cell aggregates when mixed and rotated with Dl^hs.PL-expressing cells.

(Ahimou et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in a plasmid, transfected into S2 cells and used in biochemical assays.

(Wesley, 1999)

Carried in a plasmid and transfected into S2 cells to study the effect of the Dl and wg proteins on N protein.

(Wesley and Saez, 2000)

Carried in a plasmid and transfected into S2 cells.

(Wesley and Saez, 2000)

Stably expressed in S2 cells, and used in Notch signaling assays.

(Ahimou et al., 2004)

Transfected into S2 cells and used to assay N signalling dynamics.

(Wesley and Mok, 2003)

Carried in a plasmid and used to generate stable S2 cell lines producing heatshock inducible N protein.

(Lieber et al., 1992)

N minigene encoding full length N protein, driven by a heat shock promoter.

(Lieber et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

H/N

(Lieber et al., 1992)

(Ahimou et al., 2004)

N^Hsp70Bb.PL

N^hs.PL

NFull

(Wesley and Mok, 2003)

S2-N

(Wesley, 1999)

S2-NFull

(Wesley and Mok, 2003)

hsN

(Lieber et al., 1993)

Name Synonyms

heat shock construct of Lieber

Secondary FlyBase IDs

References (13)

Report Sections

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