FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\nahar85
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General Information
Symbol
Dmel\nahar85
Species
D. melanogaster
Name
FlyBase ID
FBal0034766
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
har85
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Progenitor genotype
Cytology
Description

Nucleotide substitution: G?A. Contain a 5'-splice junction which has been altered from GTttGT to GTttaT. Also contains a 9nt deletion that changes the prredicted peptide sequence in a putative membrane-spanning segment from SDTAVTLLFTAE to SDTVLFTAE.

Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mutants are noticeably smaller than wild-type but not obviously sick or deformed. The majority of homozygous mutants that have been entrained to diurnal lighting (LD, 12hr light:12ht dark) and subsequently monitored under constant darkness (DD) are arrhythmic - only a small number are strongly rhythmic. In contrast to wild-type however, under LD entrainment however mutants exhibit most activity at night.

Show saccadic walking behavior in the absence of anaesthetics as nahar38, and abdominal morphology like nahar38. Response to enflurane and isoflurane is indistinguishable from wild type. Mosaic analysis reveals that the focus for anesthetic resistance maps close to the focus for the geotaxis defect, in the head, mapping back to the cephalic region in the blastoderm.

Homozygotes show resistance to halothane in an inebriometer assay (an assay of geotactic and postural behaviour) compared to wild-type flies.

Individuals are able to remain standing upright in the presense of halothane.

In the absence of anaesthetics flies show several mutant phenotypes. Fewer offspring are produced compared to wild type. Abdomens are more slender and elongated than wild type. Flies show hesitancy when walking, mechanical shock enhances the hesitancy. When exposed to anaesthetics individuals become immobile, they freeze, and then fall over.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Comments

nahar85 fails to complement nahar38 and other na alleles for both halothane resistance and geotactic response phenotypes.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Alleles of na form a phenotypic series, going from strongest to weakest: nahar38 > nahar85 > naP890 > na1 > naPex1 > naPex2.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (8)