FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\prodk08810
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General Information
Symbol
Dmel\prodk08810
Species
D. melanogaster
Name
FlyBase ID
FBal0035087
Feature type
allele
Associated gene
Dmel\prod
Associated Insertion(s)
P{lacW}prodk08810
Carried in Construct
Also Known As
l(2)k08810
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Torok et al., 1997)
Mutagen
P-element activity
(Torok et al., 1997, Torok et al., 1993)
Progenitor genotype
Associated Insertion(s)
P{lacW}prodk08810
Cytology
Description

Insertion of a P{lacW} element in the 5' untranslated region.

(Torok et al., 1997)
Allele components
Component
Use(s)
Inserted element
P{lacW}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygotes die as third instar larvae after a prolonged 20-day larval period. Homozygous larvae have a slower growth rate than normal, and do not reach full size until approximately 4 days after their heterozygous siblings pupariate. 9 day old larvae have tiny, slow-growing imaginal discs, which lose their epithelial organisation as the larvae age. There is massive cell death in the imaginal discs, and dead cells and cell fragments accumulate at the basal surface of the disc epithelium. Blood cells also accumulate around the discs. The first pair of lymph gland lobes begins to melanise at around 9 days after egg laying, and this melanisation spreads posteriorly as the larvae age until all the lobes are melanised and start to disintegrate. 20 day old larvae show a striking accumulation of amorphous tissue in the head region around and inside the imaginal discs, which consists of dead cell fragments and blood cells. The brain is slow-growing and smaller than normal. It develops an abnormal shape and contributes to the amorphous mass of tissue in the 20 day old larva. The penetrance of the mutant phenotype is 100%, but the expressivity is variable, with the degree of melanisation and cell death varying between different larvae. Homozygous clones (that are Minute+ in a Minute heterozygous background) in the leg have wrinkled cuticle and defective bristles. Homozygous larval tissue has a significantly lower mitotic index than wild-type, which decreases as the larvae age, until there are hardly any mitotic cells in 2 week old larvae. The metaphase/anaphase ratio in is 16:1 in mutant larval neuroblasts 5 days after egg laying, compared to 4. 6:1 in wild-type. Incomplete chromosome condensation is seen, especially near the centromeres.

      (Torok et al., 1997)

      Brain is smaller than wild type with elongated hemispheres. Discs form structureless masses, sometimes fusing with each other and the brain. The eye-antenna discs are larger than others, neoplastic outgrowths. Hyperplastic lymph glands grow as diffuse masses that fills the anterior body cavity, infrequent melanotic masses in larvae.

      (Torok et al., 1993)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      prodk08810 is rescued by prod+t8

      (Torok et al., 1997)
      Partially rescued by

      prodk08810 is partially rescued by prodUAS.cTa/Scer\GAL469B

      (Torok et al., 1997)
      Comments

      The mutant phenotype and lethality are completely rescued by prod+t8. prodScer\UAS.cTa partially rescues the mutant phenotype when expressed using Scer\GAL469B; the lethal phase shifts to the pupal or pharate adult stages, and the larvae have relatively normal imaginal discs with only slightly abnormal folding.

      (Torok et al., 1997)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      P-element excision demonstrates the insertion to be the cause of lethality.

      (Torok et al., 1993)

      Complements: l(2)0370903709.

      (BDGP Project Members, 1994-1999)

      Homozygous clones (that are Minute+ in a Minute heterozygous background) induced in larvae are recovered at a low frequency in adults and are small, indicating that prod+ function is necessary for cell proliferation and/or viability in imaginal discs and its function is cell autonomous. Germ line mosaic analysis indicates that prod+ function is required for oogenesis.

      (Torok et al., 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      l(2)k08810
      (Shim et al., 2001, Spradling et al., 1999)
      prod
      (Torok et al., 1997)
      prodk08810
      (Chavan et al., 2025, Jagannathan and Yamashita, 2021, Jagannathan et al., 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (8)