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General Information
Symbol
Dmel\Adh+t3.2
Species
D. melanogaster
Name
FlyBase ID
FBal0038104
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
rescue region
Linked to:
XbaI-XbaI restriction fragment
Comment:

The 3.2kb XhoI rescue fragment contains all of the cis-acting DNA sequences necessary for the major tissue specificities of Adh expression; position of restriction fragment on reference sequence inferred by FlyBase curator.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A 3.2kb XbaI fragment that carries the AdhF allele.

3.2kb XbaI Adh fragment which has 0.66kb of 5' flanking sequences and 0.64kb of 3' flanking sequences, all of the cis-acting DNA sequences necessary for the major tissue specificities of Adh expression.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Adh expression has correct tissue specificity but Adh protein levels of adult flies is reduced by two fold and levels in third instar larval by 10-fold.

Carried in a plasmid, expressed in E.coli cells and injected into preblastoderm embryos. Larvae and adults developed from these embryos are dissected and stained for Adh activity.

Carried in a plasmid and used in a transient expression assay.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Adh+t3.2
Name Synonyms
Secondary FlyBase IDs
  • FBal0033362
References (25)