3.2kb XbaI Adh fragment which has 0.66kb of 5' flanking sequences and 0.64kb of 3' flanking sequences, all of the cis-acting DNA sequences necessary for the major tissue specificities of Adh expression.

A 3.2kb XbaI fragment that carries the Adh^F allele.

Construct: Dpse\Hsp83 sequences from -5000 to +1258 (containing the constitutive promoter sequences up to the 5' end of exon 2) are fused in frame to the fifth codon of Tn\neoR. Hsp83 sequences from position +2402 to +2750 provide the poly(A) signal.

6.3kb SalI-BccI fragment of Dpse\Hsp83 sequences from -5000 to +1258 (containing 5kb of constitutive promoter sequences, 137bp noncoding first exon, 1063bp intron and the first 58bp of exon 2) is fused in frame to Tn\neoR. 0.34kb fragment of Hsp83 3' sequences provide the polyadenylation signal.

Dpse\Hsp83 sequences from -5000 to +1258 (containing 5000bp of non-transcribed flanking DNA with transcription regulatory elements including the promoter, the first exon, the intron and 58bp of exon 2) are fused in frame to Tn\neoR. A 340bp Hsp83 fragment provides the poly(A) signal.

Fragment of Dpse\Hsp83 sequences including 5kb of 5' flanking sequence, 137bp first exon, 1063bp intron and the first 58bp of exon 2) is fused in frame to 1.13kb segment of Tn\neoR. 0.34kb fragment of Hsp83 3' sequences provide the polyadenylation signal.