Prat^12A19/Df(3R)dsx43 animals show some lethality during the pupal stage and approximately half of the pupae contain necrotic tissue.

Wing discs from Prat^12A19/Df(3R)dsx43 white prepupae contain apoptotic cells.

Most Prat^12A19/Df(3R)dsx43 animals arrest at pupal stage P5, although some arrest at stage P10, with patches of necrotic tissue on the wing and legs.

A few Prat^12A19/Prat^16A6 and Prat^12A19/Df(3R)dsx43 adults escapers survive. Prat^12A19/Prat^16A6 and Prat^12A19/Df(3R)dsx43 female adults have a significantly shorter life span than wild-type flies. One-half of the mutant population die within the first week, while the other half show a wider distribution of life span. Heterozygous females have a reduced mean life span compared to wild-type flies, while maximum life span is almost unaffected. The progeny of Prat^12A19/Prat^16A6 or Prat^12A19/Df(3R)dsx43 females crossed to wild-type males show strongly reduced embryonic and adult survival on normal food. There is a partial rescue of this reduced survival when the food is supplemented with RNA (although the rescue of adult survival is only significant for the Prat^12A19/Df(3R)dsx43 combination), although the rate of egg production and the number of eggs produced does not differ between the two food types. Prat^12A19/Df(3R)dsx43 females show a number of egg chamber defects at stage 9-10 (58.4% of egg chambers are normal); 12.9% of egg chambers have multiple layers of follicle cells and border cell migration and localisation are abnormal, 15% have pycnotic nurse cell nuclei and the follicle cells migrate into the oocyte, 8.4% of cysts are disorganised such that the oocyte is not at the posterior pole of the egg chamber and the follicle cell layer is incomplete and 6.2% of egg chambers have an increased number of oocytes and nurse cells because two cysts are present in one egg chamber. Prat^12A19/Prat^16A6 females show a number of egg chamber defects at stage 9-10 (63.6% of egg chambers are normal); 7.9% of egg chambers have multiple layers of follicle cells and border cell migration and localisation are abnormal, 14.9% have pycnotic nurse cell nuclei and the follicle cells migrate into the oocyte, 2.2% of cysts are disorganised such that the oocyte is not at the posterior pole of the egg chamber and the follicle cell layer is incomplete and 11.4% of egg chambers have an increased number of oocytes and nurse cells because two cysts are present in one egg chamber. Embryos derived from Prat^12A19/Prat^16A6 females mated to wild-type males show defects in the early nuclear divisions, that become apparent after cycle 7. The defects seen in mutant cycle 7-13 embryos include; 17.7% of embryos show a delay in the loss of the polar body, 4.3% have chromosome bridges, 13.8% have increased yolk DNA with a normal distribution of nuclei at the blastoderm periphery, 4.6% have increased yolk DNA and nuclear gaps at the blastoderm periphery, 4.6% show desynchronisation of mitosis along the anteroposterior axis, 2.6% show desynchronisation of mitosis along the dorsoventral axis 15.7% of embryos are unfertilised (36.7% of mutant cycle 7-13 embryos appear normal).

Extremely reduced viability, and posterior crossvein gap phenotype.

Prat^12A19/Df(3R)dsx43 has partially lethal - majority die | pupal stage phenotype, suppressible | partially by BacA\p35^UAS.cHa

Prat^12A19/Df(3R)dsx43 has melanotic necrosis | pupal stage P5 phenotype, suppressible by BacA\p35^UAS.cHa

Prat^12A19/Df(3R)dsx43 has visible phenotype, suppressible by Scer\GAL4^Prat.3.8/Prat2^UAS.cPa

Prat^12A19/Df(3R)dsx43 has visible phenotype, suppressible by Scer\GAL4^{Dvir\Prat.3.9}/Prat2^UAS.cPa

Prat^12A19/Df(3R)dsx43 has wing phenotype, suppressible by Scer\GAL4^Prat.3.8/Prat2^UAS.cPa

Prat^12A19/Df(3R)dsx43 has wing phenotype, suppressible by Scer\GAL4^{Dvir\Prat.3.9}/Prat2^UAS.cPa