P-element insertion.
Males testes show the development of star shaped intranuclear crystals and needle shaped cytoplasmic crystals. Males exhibit nondisjunction and chromosome condensation defects.
Heterozygotes with cry1Y are sterile. In a search for viable deletions of Ste neither X irradiation of males, to produce sperm from gonial cells where part or all of Ste locus is removed, or Δ2-3 treatment of males, to produce deletions by imprecise excision of the insert, gave progeny when mated to virgin females. Mating to compound X cry1Y females gave 2 fertile sons, one was lost the second carried Su(Ste)+ from an exchange with the other Ste+ revertant. Lethals were recovered using Δ2-3 treatment without selecting for viability.
High resolution in situ hybridisation with a P-element probe reveals the R301.2 chromsome has two insertions, one at 12D and the other colocalises with Ste sequences at 12E1-12E2.
'X,301.2 Ste' chromosome carries 300 copies of Ste.