FlyBase Allele Report: Dmel\Ubx[Ia.UAS]

General Information

Symbol

Dmel\Ubx^Ia.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0039098

Feature type

allele

Associated gene

Dmel\Ubx

Associated Insertion(s)

Carried in Construct

P{UAS-Ubx.Ia.M}

Also Known As

UAS-Ubx

Key Links

Transgenic product class

cDNA

(Michelson, 1994)

wild_type

(Michelson, 1994)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Michelson, 1994)

wild_type

(Michelson, 1994)

Mutagen

in vitro construct

(Michelson, 1994)

Progenitor genotype

Carried in construct

P{UAS-Ubx.Ia.M}

Cytology

Description

Ubx form Ia cDNA expressed under influence of UASt regulatory sequences.

(Michelson, 1994)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ubx

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

antenna, with Scer\GAL4^upd1-E132

(Casares et al., 1996)

dorsal acute muscle cell | ectopic, with Scer\GAL4^how-24B

(Enriquez et al., 2010)

embryonic/first instar larval cuticle, with Scer\GAL4^arm.PS

(Gebelein et al., 2002)

embryonic abdominal segment 1, with Scer\GAL4⁴⁴⁴

(Azpiazu and Morata, 1998)

embryonic abdominal segment 1 & abdominal ventral oblique muscle 6 | ectopic, with Scer\GAL4^how-24B

(Michelson, 1994)

embryonic abdominal segment 1 & abdominal ventral transverse muscle 1 | ectopic, with Scer\GAL4^how-24B

(Michelson, 1994)

embryonic mesothoracic segment, with Scer\GAL4⁴⁴⁴

(Azpiazu and Morata, 1998)

embryonic metathoracic segment, with Scer\GAL4⁴⁴⁴

(Azpiazu and Morata, 1998)

embryonic midgut constriction 1, with Scer\GAL4^how-24B

(Michelson, 1994)

embryonic prothoracic segment, with Scer\GAL4⁴⁴⁴

(Azpiazu and Morata, 1998)

embryonic thoracic segment & ventral longitudinal muscle, with Scer\GAL4^how-24B

(Michelson, 1994)

gastric caecum, with Scer\GAL4^how-24B

(Michelson, 1994)

indirect flight muscle cell, with Scer\GAL4¹¹⁵¹

(Roy and VijayRaghavan, 1997)

Keilin organ, with Scer\GAL4^arm.PS

(Gebelein et al., 2002)

muscle cell of mesothoracic dorsal acute muscle 3, with Scer\GAL4^{kirre-rP298-G4}

(Enriquez et al., 2010)

muscle cell of metathoracic dorsal acute muscle 3, with Scer\GAL4^{kirre-rP298-G4}

(Enriquez et al., 2010)

scutum, with Scer\GAL4^C-765

(Casares et al., 1996)

thoracic segment & abdominal ventral acute muscle 1 | precursor | ectopic, with Scer\GAL4^how-24B

(Michelson, 1994)

ventral denticle belt, with Scer\GAL4^arm.PS

(Gebelein et al., 2002)

wing, with Scer\GAL4^Bx-MS1096

(Casares et al., 1996)

wing, with Scer\GAL4^C-765

(Casares et al., 1996)

Detailed Description

Statement

Reference

Scer\GAL4^how-24B-mediated expression of Ubx^Ia.Scer\UAS results in an ectopic DA3 muscle in thoracic segment 1. The number of nuclei in this ectopic muscle is identical to that in wild type abdominal segments.

Expression of Ubx^Ia.Scer\UAS using Scer\GAL4^kirre-rP298, but not Scer\GAL4^sns.PK, converts the number of nuclei in thoracic segments 2 and 3 to that found in abdominal segments.

(Enriquez et al., 2010)

85% of the embryonic/first instar larval cuticles of Ubx^Ia.Scer\UAS; Scer\GAL4^arm.PS animals lack Keilin's organs.

(Gebelein et al., 2002)

Scer\GAL4⁴⁴⁴-mediated expression at 17^oC causes the thoracic and some head segments of first instar larvae to display A1 identity. At 29^oC larvae develop all segments anterior to A2 with an A3-A5 pattern.

(Azpiazu and Morata, 1998)

When expressed via Scer\GAL4¹¹⁵¹, T2 myoblast migration during pupal development is unaffected. The indirect flight muscle fail to complete development and eventually degenerate.

(Roy and VijayRaghavan, 1997)

Scer\GAL4^E132 induced expression in the eye-antennal disc causes transformation of the antenna into leg. Scer\GAL4^Bx-MS1096 or Scer\GAL4^C-765 induced expression in the wing disc causes transformation of the wing into haltere-like tissue. Co-expression of P{UAS-exd.G} (induced by Scer\GAL4^Bx-MS1096) does not significantly modify the wing transformation. Scer\GAL4^C-765 induced expression also transforms the mesonotum into the homologous region of the third thoracic segment. Scer\GAL4^c739 and Scer\GAL4^C-765 induced expression in the abdomen fails to cause segmental change. Ubx^abx-1 Ubx^bx-3 Ubx^pbx-1/Ubx^lac1 combination transforms the metathorax into mesothorax (including transformation of halteres into wings), Scer\GAL4^Bx-MS1096 induced expression allows full rescue.

(Casares et al., 1996)

When expressed via Scer\GAL4^how-24B in embryos, an ectopic precursor for abdominal muscle 26 appears in the ventral region of T1-T3. Ventral longitudinal muscles of thoracic segments assume the identity of the abdominal segments. Abdominal segment 1 gains two muscles that it normally lacks: external ventral oblique muscles 17 and 25. Insertion sites of transformed muscles are sometimes aberrant. The thoracic cuticle remains unaffected. Both gastric caeca and the first midgut constriction are missing.

(Michelson, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Phenotype can be partially reverted by adding an additional dose of exd⁺.

(Azpiazu and Morata, 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Expression dependent on Scer\GAL4.

(Michelson, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ubx^Ia.Scer\UAS

Ubx^Ia.UAS

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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