FlyBase Allele Report: Dmel\f[+t13]

General Information

Symbol

Dmel\f^+t13

Species

D. melanogaster

Name

FlyBase ID

FBal0039342

Feature type

allele

Associated gene

Dmel\f

Associated Insertion(s)

Carried in Construct

P{f⁺13}

Key Links

Transgenic product class

genomic_DNA

(Petersen et al., 1994)

wild_type

(Petersen et al., 1994)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Petersen et al., 1994)

wild_type

(Petersen et al., 1994)

Mutagen

in vitro construct

(Tilney et al., 1998, Petersen et al., 1994)

Progenitor genotype

Carried in construct

P{f⁺13}

Cytology

Description

13kb NotI-SalI fragment from the genomic cosmid clone CoSpeR-forked-12-2, including the coding regions for the 2.5kb, 1.9kb and 1.1kb f transcripts.

(Petersen et al., 1994)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Duboff et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Tilney et al., 2000, Tilney et al., 1998)

wild-type

(Petersen et al., 1994)

Phenotype Manifest In

adult brain

(Duboff et al., 2012)

macrochaeta

(Tilney et al., 1998)

macrochaeta & actin filament

(Tilney et al., 1998)

microchaeta

(Tilney et al., 2004, Tilney et al., 1998)

microchaeta & actin filament

(Tilney et al., 2003, Tilney et al., 2000)

mitochondrion

(Duboff et al., 2012)

Detailed Description

Statement

Reference

The mitochondria in the neurons of adult brains expressing f^+t13 are frequently elongated compared to controls.

(Duboff et al., 2012)

Flies that carry 4 copies of the f^+t13 transgene have bristles that are shorter and straighter than in wild type, but are curved at the tip.

(Tilney et al., 2004)

Bristle cells with 4 copies of the f^+t13 transgene form longitudinal actin bundles with an isosceles triangle shape that project deep into the cytoplasm and contain more filaments than wild type.

(Tilney et al., 2003)

Flies carrying four copies of f^+t13 in a f⁺ background often have very large actin bundles in the bristles and these bundles are often triangular in shape. Smaller bundles of filaments are also seen in the cytoplasm and some of them are connected on one of their surfaces to the large triangular-shaped peripheral bundles. The filaments in these bundles are hexagonally packed. The area occupied by the bundles is larger than in wild type (15% vs. 7.5%).

(Tilney et al., 2000)

Macrochaetae and some microchaetae of flies carrying four copies of f^+t13 in a f⁺ background often have a fish hook appearance. Actin bundles in the barb portion of the bristle are very large and often irregular in shape. In addition to the membrane-associated actin bundles there are also internal bundles composed of hexagonally packed filaments, which tend to aggregate together on one half of the bristle shaft. Many of the membrane-associated bundles appear to be composed of closely applied subbundles each containing approximately 50-100 filaments.

(Tilney et al., 1998)

Increasing the copy number of f^+t13 induces a hypermorphic bristle phenotype whose severity correlates with the number of copies of the alleles present.

(Petersen et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

f^+t13 is an enhancer of adult brain phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

f^+t13 is an enhancer of mitochondrion phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of f^+t13 enhances the increase in mitochondria length seen when Hsap\MAPT^{R406W.Scer\UAS} is expressed under the control of Scer\GAL4^elav.PU.

(Duboff et al., 2012)

Complementation and Rescue Data

Rescues

f^+t13 rescues f^hd

(Petersen et al., 1994)

f^+t13 rescues f^36a

(Petersen et al., 1994)

Comments

Images (0)

Mutant

Wild-type

Stocks (15)