FlyBase Allele Report: Dmel\gsb[hs.PZ]

General Information

Symbol

Dmel\gsb^hs.PZ

Species

D. melanogaster

Name

heat shock construct of Zhang

FlyBase ID

FBal0039380

Feature type

allele

Associated gene

Dmel\gsb

Associated Insertion(s)

Carried in Construct

P{hs-gsb-d.Z}

Also Known As

gsb-d^hs.PZ

Key Links

Transgenic product class

genomic_DNA

(Zhang et al., 1994)

wild_type

(Zhang et al., 1994)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Zhang et al., 1994)

wild_type

(Zhang et al., 1994)

Mutagen

in vitro construct

(Zhang et al., 1994)

Progenitor genotype

Carried in construct

P{hs-gsb-d.Z}

Cytology

Description

Genomic fragment containing entire coding region of gsb, driven by an Hsp70 promoter.

(Zhang et al., 1994)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

gsb

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | heat sensitive

(Zhang et al., 1994)

wild-type

(Zhang et al., 1994)

Phenotype Manifest In

embryonic central nervous system & neuroblast | conditional ts

(Zhang et al., 1994)

embryonic epidermis | heat sensitive

(Zhang et al., 1994)

Keilin organ | heat sensitive

(Zhang et al., 1994)

larval DA1 motor neuron | heat sensitive

(Zhang et al., 1994)

neuroblast MP2

(Skeath et al., 1995)

pCC neuron | heat sensitive

(Zhang et al., 1994)

Detailed Description

Statement

Reference

Ectopic expression in the embryonic neuroectoderm before neuroblast formation causes a neuroectodermal phenotype, row 3 neuroblasts are transformed to row 5. There is little effect on rows 1 and 7. Ectopic expression after neuroblast formation but before its division does not cause transformation.

(Skeath et al., 1995)

Groups of denticles or entire denticle belts are replaced with naked cuticle. Heat shocks from 2.75 to 3.25 hours transform complete segments while those after 4 hours transform parts of segments. Multiple heat shocks generate embryos with completely naked cuticles. Ectopic Keilin's organs sometimes develop. Row three neuroblasts of the embryonic CNS are transformed into row 5 neuroblasts. The CNS also shows fusion of two commissures in each segment and the elimination of the longitudinal connectives. aCC and pCC are eliminated at higher frequency than for gsb-n^hs.PZ.

(Zhang et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Prevents the duplication of RP2 neurons seen in en^E inv^E embryos when expressed during stage 8.

(Bhat and Schedl, 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

gsb^hs.PZ rescues gsb^IIX62

(Zhang et al., 1994)

Comments

A series of three heat shocks almost completely rescues the mutant Df(2R)gsb cuticle phenotype.

(Zhang et al., 1994)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

gsb-d^hs.PZ

(Skeath et al., 1995, Zhang et al., 1994)

gsb^hs.PZ

Name Synonyms

heat shock construct of Zhang

(Zhang et al., 1994)

Secondary FlyBase IDs

References (3)

Report Sections

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