FlyBase Allele Report: Ecol\lacZ[eve.8.0]

General Information

Symbol

Ecol\lacZ^eve.8.0

Species

E. coli

Name

FlyBase ID

FBal0041342

Feature type

allele

Associated gene

Ecol\lacZ

Associated Insertion(s)

Carried in Construct

P{eve-lacZ8.0}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Cai and Levine, 1995, Zhong et al., 1993, Panzer et al., 1992, Small et al., 1992, Harding et al., 1989)

Progenitor genotype

Ecol\lacZ^eve.5.2

(Harding et al., 1989)

Carried in construct

P{eve-lacZ8.0}

Cytology

Description

8kb fragment of eve promoter sequences, including the enhancer for stripe 2 and 3 eve expression, drives expression of lacZ.

(Small et al., 1992)

Insertion of a 5' restriction fragment resulting in 8.0kb of eve promoter sequences fused to lacZ.

(Harding et al., 1989)

Allele components

Component

Use(s)

Regulatory region(s)

eve_8.0-lacZ

Encoded product / tool

lacZ

reporter enzyme

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 17

organism | striped

(Harding et al., 1989)

embryonic stage 9 -- 17

organism | pair rule expression pattern

(Harding et al., 1989)

Additional Information

Statement

Reference

In cellularizing embryos, EcollacZ expression is observed, but differs from normal eve expression. Stripes 2, 3, and 7 appear normal, stripe 1 is reduced in expression, and stripes 4, 5, and 6 are undetectable. The missing stripes start to appear during gastrulation. By extended germband stage, EcollacZ expression corresponds to normal 7-stripe eve pattern.

(Harding et al., 1989)

Marker for

Reflects expression of

eve

(Harding et al., 1989)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References