FlyBase Recombinant Construct Report: P{eve-lacZ8.0}

General Information

Symbol

P{eve-lacZ8.0}

FlyBase ID

FBtp0000391

Feature type

transgenic_transposable_element

Component Allele(s)

Ecol\lacZ^eve.8.0

Size

Expression Data

lacZ reporter

Associated insertion(s)

4 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

ry^+t7.2

Component allele Ecol\lacZ^eve.8.0

Transgenic product class

Component

Use(s)

Regulatory region(s)

eve_8.0-lacZ

Encoded product / tool

lacZ

reporter enzyme

Description

Insertion of a 5' restriction fragment resulting in 8.0kb of eve promoter sequences fused to lacZ.

(Harding et al., 1989)

8kb fragment of eve promoter sequences, including the enhancer for stripe 2 and 3 eve expression, drives expression of lacZ.

(Small et al., 1992)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Ecol\lacZ^eve.8.0

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 17

organism | striped

(Harding et al., 1989)

embryonic stage 9 -- 17

organism | pair rule expression pattern

(Harding et al., 1989)

Additional Information

Statement

Reference

In cellularizing embryos, EcollacZ expression is observed, but differs from normal eve expression. Stripes 2, 3, and 7 appear normal, stripe 1 is reduced in expression, and stripes 4, 5, and 6 are undetectable. The missing stripes start to appear during gastrulation. By extended germband stage, EcollacZ expression corresponds to normal 7-stripe eve pattern.

(Harding et al., 1989)

Marker for

Reflects expression of

eve

(Harding et al., 1989)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{eve-lacZ8.0}

(Harding et al., 1989)

descendant(s)