FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Ecol\lacZnanos.3UTR.Hsp83
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General Information
Symbol
Ecol\lacZnanos.3UTR.Hsp83
Species
E. coli
Name
FlyBase ID
FBal0041803
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{lacZ-nanos3'UTR}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Gavis et al., 1996, Gavis and Lehmann, 1992)
Progenitor genotype
Ecol\lacZC4.Adh
(Gavis et al., 1996)
Carried in construct
P{lacZ-nanos3'UTR}
Cytology
Description

Hsp83 promoter drives expression of a 4.3kb EcoRI fragment containing Adh leader sequences, lacZ coding sequences and SV40 t antigen splice site and polyadenylation signal (Ecol\lacZC4.Adh). nos 3' UTR fragment from nucleotide 6 to 859 is inserted between the lacZ coding sequence and the SV40 sequences.

(Gavis et al., 1996)

An Hsp83 promoter drives expression of lacZ. A 866bp fragment of the nos 3'UTR is fused downstream of the lacZ coding sequence.

(Gavis and Lehmann, 1992)
Allele components
Component
Use(s)
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      No Assay Recorded
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      primordial germ cell
      (Gavis and Lehmann, 1992)
      in situ
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      blastoderm stage
      organism | 0-10% egg length
      (Gavis and Lehmann, 1992)
      Additional Information
      Statement
      Reference

      EcollacZ transcripts containing the 3'UTR of nos are tightly localized to the posterior pole of early embryosand are later incorporated into pole cells just like wild type nostranscripts.

      (Gavis and Lehmann, 1992)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ecol\lacZnanos.3UTR.Hsp83
      Ecol\lacZnos.3UTR.Hsp83
      lacZnos.3UTR.Hsp83
      Name Synonyms
      Secondary FlyBase IDs
        References (3)