FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{lacZ-nanos3'UTR}
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General Information
Symbol
P{lacZ-nanos3'UTR}
FlyBase ID
FBtp0001617
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZnanos.3UTR.Hsp83
Size
Expression Data
lacZ reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Ecol\lacZnanos.3UTR.Hsp83
Transgenic product class
Component
Use(s)
Encoded product / tool
lacZ
Description

An Hsp83 promoter drives expression of lacZ. A 866bp fragment of the nos 3'UTR is fused downstream of the lacZ coding sequence.

(Gavis and Lehmann, 1992)

Hsp83 promoter drives expression of a 4.3kb EcoRI fragment containing Adh leader sequences, lacZ coding sequences and SV40 t antigen splice site and polyadenylation signal (Ecol\lacZC4.Adh). nos 3' UTR fragment from nucleotide 6 to 859 is inserted between the lacZ coding sequence and the SV40 sequences.

(Gavis et al., 1996)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
primordial germ cell
(Gavis and Lehmann, 1992)
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
blastoderm stage
organism | 0-10% egg length
(Gavis and Lehmann, 1992)
Additional Information
Statement
Reference

EcollacZ transcripts containing the 3'UTR of nos are tightly localized to the posterior pole of early embryosand are later incorporated into pole cells just like wild type nostranscripts.

(Gavis and Lehmann, 1992)
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

construct_comment: Progenitors are those of the plasmid, pP{lacZ-nos3\'UTR}.

(Gavis and Lehmann, 1992)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{lacZ-nanos3'UTR}
(FlyBase, 1996-)
P{lacZ-nos3'UTR}
(FlyBase, 1996-)
l-n3''UTR
(Gavis and Lehmann, 1992)
Secondary FlyBase IDs
    References (5)