FlyBase Allele Report: Dmel\ftz[hs.PS]

General Information

Symbol

Dmel\ftz^hs.PS

Species

D. melanogaster

Name

heat shock construct of Struhl

FlyBase ID

FBal0043085

Feature type

allele

Associated gene

Dmel\ftz

Associated Insertion(s)

Carried in Construct

P{hsp70-ftz}

Also Known As

hsf2

Key Links

Transgenic product class

wild_type

(Struhl, 1985)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Struhl, 1985)

Mutagen

in vitro construct

(Welte et al., 1995, Ish-Horowicz et al., 1989, Ish-Horowicz and Gyurkovics, 1988, Struhl, 1985)

Progenitor genotype

Carried in construct

P{hsp70-ftz}

Cytology

Description

A 450bp fragment of the Hsp70 promoter region drives the expression of the ftz open reading frame (composed of genomic and cDNA).

(Struhl, 1985)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

ftz

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | heat sensitive

(Hughes and Krause, 2001)

pair rule phenotype

(Alonso et al., 2001, Struhl, 1985)

pair rule phenotype | heat sensitive

(Hughes and Krause, 2001)

Phenotype Manifest In

abdominal 2 ventral denticle belt

(Struhl, 1985)

abdominal 4 ventral denticle belt

(Struhl, 1985)

abdominal 6 ventral denticle belt

(Struhl, 1985)

abdominal 8 ventral denticle belt

(Struhl, 1985)

embryo

(Alonso et al., 2001)

embryo | heat sensitive

(Hughes and Krause, 2001)

embryonic/first instar larval cuticle | heat sensitive

(Hughes and Krause, 2001)

embryonic abdomen

(Ish-Horowicz and Gyurkovics, 1988)

embryonic segment | heat sensitive

(Hughes and Krause, 2001)

embryonic thorax

(Ish-Horowicz and Gyurkovics, 1988)

metathoracic ventral denticle belt

(Struhl, 1985)

parasegment

(Alonso et al., 2001)

parasegment 3 | heat sensitive

(Hughes and Krause, 2001)

parasegment 4 | heat sensitive

(Hughes and Krause, 2001)

parasegment 5 | heat sensitive

(Hughes and Krause, 2001)

parasegment 6 | heat sensitive

(Hughes and Krause, 2001)

prothoracic ventral denticle belt

(Struhl, 1985)

segment

(Struhl, 1985)

Detailed Description

Statement

Reference

Expression of ftz^hs.PS using heat shock results in pair-rule defects in 36% of embryos; odd-numbered parasegments are eliminated.

(Alonso et al., 2001)

Expression of ftz^hs.PS using heat shock from 2.25-2.75 hours after egg laying results in a pair rule phenotype. The segments that remain are about 1.4 times wider than wild-type segments and the cuticle is about 70% the length of a wild-type cuticle. The number of embryos that hatch and the number of adults eclosing is dramatically reduced compared to controls. Parasegments 3 to 6 are unevenly spaced at stage 6-7 with parasegments 4 and 6 being about 1.4 times wider than wild-type and parasegments 3 and 5 being about 0.6 times the wild-type width. The wider parasegments contain about twice the number of cells of the narrower parasegments. The total width and number of cells in all four parasegments is equivalent to wild type. Each of the narrow parasegments maintains a constant relative width until around stage 12. After this time they decrease further in both width and cell number. The wide parasegments remain about 1.3-1.5 times wider and contain about 1.4 times as many cells as a normal parasegment.

(Hughes and Krause, 2001)

Ectopic expression between 2.5 and 3 hours of development causes pair rule deletion in the pattern of cuticular structures formed at the end of embryogenesis.

(John et al., 1995)

Ectopic ftz expression causes severe pattern defects.

(Ish-Horowicz et al., 1989)

The dorsal pattern of embryos in which ftz^hs.PS has been expressed during the blastoderm stage using heat shock indicates that the apparent segments have a mixed segmental character. The 'A1-like' segment includes the posterior row of dorsal hairs characteristic of pA2 (posterior second abdominal segment). The anterior band of dorsal hairs in the 'T2-like' segment extends past the dorsal sense organs as is usually found in pT3 (posterior third thoracic segment).

(Ish-Horowicz and Gyurkovics, 1988)

Ectopic expression between 2.5 and 3.5 hours of development causes a pair rule phenotype. Larvae show deletion of even numbered segments. Heat induction after blastoderm stage or during embryogenesis does not give a pair rule phenotype.

(Struhl, 1985)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ftz^hs.PS has lethal | heat sensitive phenotype, suppressible by eve^hs.PS

(Hughes and Krause, 2001)

Suppressor of

Statement

Reference

ftz^hs.PS is a suppressor of lethal | heat sensitive phenotype of eve^hs.PS

(Hughes and Krause, 2001)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of ftz^hs.PS and eve^hs.PS results in a return to viability near to control levels, both for the numbers of embryos that hatch and the number of adults eclosing.

(Hughes and Krause, 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

3523

P{hsp70-ftz}HSF2, Adh^fn6 cn¹ bw¹/CyO, Adh^*; ry^*

Notes on Origin

Discoverer

Struhl.

(Welte et al., 1995)

Comments

Carried in plasmid "pHF1" and expressed in Schneider line S2 cells.

(Welte et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ftz^hs.PS

Name Synonyms

heat shock construct of Struhl

(Struhl, 1985)

Secondary FlyBase IDs

References (10)

Report Sections

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