FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Ecol\lacZFRT.Act5C.cBa.Tag:NLS(Unk)
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General Information
Symbol
Ecol\lacZFRT.Act5C.cBa.Tag:NLS(Unk)
Species
E. coli
Name
FlyBase ID
FBal0043853
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{Act5C(FRT.polyA)lacZ.nls1}
Also Known As
act5c>stop>lacZ, Actin5C FRT-stop-FRT lacZ-nls, Act>DRaf>lacZ, act5C-FRT-stop-FRT-lacZ
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Campbell and Tomlinson, 1998, Park et al., 1998, Okabe and Okano, 1997, Reddy et al., 1997, Struhl and Basler, 1993)
Progenitor genotype
Carried in construct
P{Act5C(FRT.polyA)lacZ.nls1}
Cytology
Description

Act5C promoter is positioned upstream of a coding sequence for a nuclear localized lacZ reporter, but separated by an FRT cassette containing an Hsp70 transcriptional stop. The FRT cassette also includes a 4.4kb fragment containing RafC20.

(Struhl and Basler, 1993)
Allele components
Component
Use(s)
Regulatory region(s)
Act5C
Encoded product / tool
lacZ
Tagged with
Tag:NLS(Unk)
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference

      Designed for use in mosaic analyses; transcription blocked by FRT-flanked fragment that contains Hsp70 polyA site and 4.4kb of RafC20 sequences. See expression pattern for @EcollacZ<up>Act5C.PS. T:Zzzz

      ls1</up>RA, carried in descendant transposon FBtp0001102:P {Act5C(-FRT)lacZ.nls1}@.

      (Struhl and Basler, 1993)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (3)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (6)
      Reported As
      Symbol Synonym
      Act5C>Draf+>nuc-lacZ
      (Manfruelli et al., 1999)
      Ecol\lacZFRT.Act5C.cBa.Tag:NLS(Unk)
      Ecol\lacZScer\FRT.Act5C.T:Zzzz\nls-KKKRKV
      Ecol\lacZScer\FRT.Act5C.T:Zzzz\nls1
      Ecol\lacZScer\FRT.Act5C.cBa.T:Uuuu\nls-Unk
      lacZFRT.Act5C.T:nls1
      Name Synonyms
      Secondary FlyBase IDs
        References (37)