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General Information
Symbol
P{Act5C(FRT.polyA)lacZ.nls1}
FlyBase ID
FBtp0001103
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
act5c>stop>lacZ, Act>DRaf>lacZ, act5C-FRT-stop-FRT-lacZ, Actin5C FRT-stop-FRT lacZ-nls
Size
Expression Data
lacZ reporter
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
Construct components
Product class / Tool use(s)
Marker(s)
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Description

Act5C promoter is positioned upstream of a coding sequence for a nuclear localized lacZ reporter, but separated by an FRT cassette containing an Hsp70 transcriptional stop. The FRT cassette also includes a 4.4kb fragment containing RafC20.

Component allele RafC20
Product class / Tool use(s)
Regulatory region(s)
Description

Contained within a 4.4kb BamHI fragment that includes the entire phl+ gene.

4.3kb BamHI fragment of the Raf region.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
removable
cassette
removable
element
selectable
marker
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference

Designed for use in mosaic analyses; transcription blocked by FRT-flanked fragment that contains Hsp70 polyA site and 4.4kb of RafC20 sequences. See expression pattern for Ecol\lacZAct5C.PS.T:Zzzz\nls1RA, carried in descendant transposon P{Act5C(-FRT)lacZ.nls1}.

 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

report comment: lacZ allele contains a nuclear localization signal.Allele lacZAct5C.PS.T:nls is the FRT-deleted (active) form, carried on descendant transposon P{lacZ.Act5C.S}.

Stocks (3)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (17)
Reported As
Symbol Synonym
Act5C FRT Draf+ FRT nuc-lacZ
Act5CFRTDraf+FRTnuc-lacZ
Act5Cfrt-stop-frt-LacZ
Act>Draf>lacZ
Actin5C FRT-stop-FRT lacZ-nls
Actin5C>raf+nuc-lacZ
Actin>stop>lacZ
P{Act5C(FRT.polyA)lacZ.nls1}
P{lacZ.FRT.S}
act5c>stop>lacZ
actin-FRT-Draf+-FRT-nuc.lacZ
Secondary FlyBase IDs
    References (32)