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Citation
Morrison, C.M., Halder, G. (2010). Characterization of a Dorsal-Eye Gal4 Line in Drosophila.  genesis 48(1): 3--7.
FlyBase ID
FBrf0209713
Publication Type
Research paper
Abstract

In Drosophila, the Gal4-UAS system is used to drive ectopic gene expression in a tissue-specific manner. In this system, transgenic flies expressing tissue specific Gal4 are crossed to a line in which the gene to be expressed is under the control of a Gal4-responsive UAS sequence. The resulting progeny express the gene of interest in the pattern of the particular Gal4 line. Since a given UAS-transgene can be driven by any Gal4 line, this system is predominantly limited by available Gal4 lines. Here we report the characterization of a novel line, DE-Gal4, which in the eye is expressed in the dorsal compartment for the majority of development. Furthermore, we use functional tests to show that the DE-Gal4 line is a useful tool with which to manipulate gene expression in half of the developing eye.

PubMed ID
PubMed Central ID
PMC3162325 (PMC) (EuropePMC)
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Secondary IDs
  • FBrf0209697
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
genesis
Title
genesis
Publication Year
2000-
ISBN/ISSN
1526-954X 1526-968X
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