put⁸⁸ embryos raised at the permissive temperature until stage 14, then switched to the restrictive temperature show axon guidance defects; the ISNb axons stall in 31% of hemisegments and 32% of SNa axons fail to branch or extend completely.

At 25^oC, 39% of homozygous embryos have a strong dorsal-open phenotype (large holes in the dorsal cuticle), 31% have an intermediate phenotype (the dorsal cuticle is noticeably reduced, and there is increased curvature in the abdominal segments, resulting in a "tail-up" phenotype) and 31% have a weak phenotype. put¹⁰/put⁸⁸ adults are viable, and sometimes show defects in wing venation. Temperature shift experiments using put¹³⁵/put⁸⁸ animals indicate that there is an absolute requirement for zygotic put function between 8 and 12 hours of embryogenesis.

Heterozygotes with put¹⁰⁴⁶⁰ are lethal at high temperatures, at low temperatures individuals survive and exhibit ectopic wing venation in the region of the posterior cross vein. Escaper females produce ventralised embryos.