FlyBase Allele Report: Btau\MAPT[UAS.cKa]

General Information

Symbol

Btau\MAPT^UAS.cKa

Species

B. taurus

Name

Saccharomyces cerevisiae UAS construct a of Kerscher

FlyBase ID

FBal0044378

Feature type

allele

Associated gene

Btau\MAPT

Associated Insertion(s)

Carried in Construct

P{UAS-btau.K}

Also Known As

UAS-tau, UAS-btau

Transgenic product class

cDNA

(Ito et al., 1997)

wild_type

(Ito et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Ito et al., 1997)

wild_type

(Ito et al., 1997)

Mutagen

in vitro construct

(Heimbeck et al., 2001, Ito et al., 1997, Ito et al., 1997)

Progenitor genotype

Carried in construct

P{UAS-btau.K}

Cytology

Description

UASt regulatory sequences drive expression of a 1.7kb EcoRI fragment of Btau\MAPT cDNA.

(Ito et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Btau\MAPT

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning, with Scer\GAL4^c492b

(Mershin et al., 2004)

abnormal learning, with Scer\GAL4^c747

(Mershin et al., 2004)

abnormal learning, with Scer\GAL4^elav.PU

(Kosmidis et al., 2010, Papanikolopoulou et al., 2010)

abnormal memory, with Scer\GAL4^c492b

(Mershin et al., 2004)

abnormal memory, with Scer\GAL4^c747

(Mershin et al., 2004)

abnormal smell perception, with Scer\GAL4^elav.PU

(Kosmidis et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

The mushroom bodies in flies expressing Btau\MAPT^Scer\UAS.cKa in neurons under the control of Scer\GAL4^elav.PU appear normal.

The associative olfactory learning performance of animals expressing Btau\MAPT^Scer\UAS.cKa under the control of Scer\GAL4^elav.PU is highly deficient compared with controls.

Animals expressing Btau\MAPT^Scer\UAS.cKa under the control of Scer\GAL4^elav.PU display altered olfactory response to octanol and benzaldehyde compared with controls.

(Kosmidis et al., 2010)

The associative olfactory learning performance of animals expressing Btau\MAPT^Scer\UAS.cKa using Scer\GAL4^elav.PU appears impaired without any obvious defects in the structural integrity of mushroom bodies compared with controls.

(Papanikolopoulou et al., 2010)

Expression of Btau\MAPT^Scer\UAS.cKa in the adult mushroom body and other brain neurons under the control of either Scer\GAL4^c492b or Scer\GAL4^c747 does not result in decreased longevity. Btau\MAPT^Scer\UAS.cKa-expression does not appear to cause gross morphological abnormalities or decreased fecundity and vigor compared with controls. Degenerating cells and large vacuoles are not detected in the mushroom bodies of controls or 21 day old animals expressing Btau\MAPT^Scer\UAS.cKa.

The performance of flies expressing Btau\MAPT^Scer\UAS.cKa under the control of either Scer\GAL4^c492b or Scer\GAL4^c747 is not significantly different from controls in negatively reinforced associative olfactory learning tasks.

The avoidance-response to electrified grids kept at 90 V or 15 V is not different between animals expressing Btau\MAPT^Scer\UAS.cKa under the control of either Scer\GAL4^c492b or Scer\GAL4^c747 and controls. Btau\MAPT^Scer\UAS.cKa-expressing animals and controls exhibit equal decrements in response to olfactory conditioned stimuli after experiencing couples conditioned plus unconditioned stimuli.

Scer\GAL4^c747>Btau\MAPT^Scer\UAS.cKa and Scer\GAL4^c492b>Btau\MAPT^Scer\UAS.cKa flies exhibit a highly significant impairment in learning compared with controls. The memory of the conditioned association 90 minutes later is significantly depressed in Btau\MAPT^Scer\UAS.cKa-expressing animals, and to a lesser degree at 180 minutes.

Conditioned/unconditioned pairing in Scer\GAL4^c747>Btau\MAPT^Scer\UAS.cKa-expressing flies yields olfactory learning scores nearly 50% lower than those of controls. The Btau\MAPT^Scer\UAS.cKa-expressing animals exhibit a significant decrease in memory retention after 30 minutes, despite performing at equivalent level with controls immediately after training.

(Mershin et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Btau\MAPT^UAS.cKa is a suppressor | partially of neuropil phenotype of futsch^olk1

(Bettencourt da Cruz et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Flies that express Btau\MAPT^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 show a 25% reduction in the size of vacuoles that develop in the mechanosensory neuropil compared to futsch^olk1 flies that do not express the transgene.

(Bettencourt da Cruz et al., 2005)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Axonal staining.

(Kerscher et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Btau\MAPT^Scer\UAS.cKa

Btau\MAPT^UAS.cKa

btau^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kerscher

(Kerscher et al., 1995)

Secondary FlyBase IDs

FBal0057726

References (26)

Report Sections

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