Sgs3 coding sequence and 127bp of upstream sequences.
Construct: Contains a SalI-XhoI fragment from the 7kb genomic fragment present in Sgs3e.g1. Contains Sgs3e sequence from -127bp (relative to the presumed cap site) through to 320bp downstream of the polyadenylation signal.
Role of Gebf-I binding in the Sgs3 enhancer activity is studied.