FlyBase Allele Report: Dmel\gcm[UAS.cHa]

General Information

Symbol

Dmel\gcm^UAS.cHa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Hosoya

FlyBase ID

FBal0045764

Feature type

allele

Associated gene

Dmel\gcm

Associated Insertion(s)

Carried in Construct

P{UAS-gcm.H}

Also Known As

UAS-gcm

Key Links

Transgenic product class

cDNA

(Hosoya et al., 1995)

wild_type

(Hosoya et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Hosoya et al., 1995)

wild_type

(Hosoya et al., 1995)

Mutagen

in vitro construct

(Akiyama-Oda et al., 1998, Hosoya et al., 1995)

Progenitor genotype

Carried in construct

P{UAS-gcm.H}

Cytology

Description

UASt regulatory sequences drive expression of a MluI-AfIII fragment of gcm cDNA (contains the entire coding region plus about 100bp of 5' and 200bp of 3' untranslated sequence).

(Hosoya et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

gcm

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^sca-T3

(Yuasa et al., 2003)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of gcm^Scer\UAS.cHa under the control of Scer\GAL4^sca-T3 results in a reduction in the number of elav-positive neurons and axonal extension in embryos.

(Yuasa et al., 2003)

The central nervous system is markedly disorganised in embryos expressing gcm^Scer\UAS.cHa under the control of Scer\GAL4^sca-537.4.

(Soustelle et al., 2002)

Stage 13-15 embryos expressing gcm^Scer\UAS.cHa under the control of Scer\GAL4^en-e16E have extra glial cells and do not have neurons of the NB6-4T lineage.

(Akiyama-Oda et al., 1999)

Embryonic epidermal cells expressing gcm^Scer\UAS.cHa under the control of Scer\GAL4^en-e16E move from the surface cell layer towards the inside of the body and are mesenchymal in morphology in contrast to the columnar shape of normal epithelial cells. Embryos expressing gcm^Scer\UAS.cHa under the control of Scer\GAL4^69B are severely malformed and show defects in dorsal closure. Embryos expressing gcm^Scer\UAS.cHa under the control of Scer\GAL4^twi.PG show severe disruption of the somatic muscles; although muscle fibres are formed, they are very reduced in number compared with controls.

(Akiyama-Oda et al., 1998)

Scer\GAL4-mediated expression transforms most neurons into glia.

(Iwai et al., 1997)

Ectopic expression of gcm inhibits neuronal differentiation while promoting glial differentiation.

(Hosoya et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^sca-T3, gcm^UAS.cHa has abnormal neuroanatomy phenotype, suppressible by repo⁶⁴

(Yuasa et al., 2003)

NOT suppressed by

Statement

Reference

Scer\GAL4^sca-T3, gcm^UAS.cHa has abnormal neuroanatomy phenotype, non-suppressible by pnt^Δ88

(Yuasa et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^sca-T3, gcm^UAS.cHa has larval neuron phenotype, suppressible by repo⁶⁴

(Yuasa et al., 2003)

NOT suppressed by

Statement

Reference

Scer\GAL4^sca-T3, gcm^UAS.cHa has larval neuron phenotype, non-suppressible by pnt^Δ88

(Yuasa et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos expressing gcm^Scer\UAS.cHa under the control of Scer\GAL4^sca-T3 show a dramatic restoration of elav-positive cells and neuronal development if they are also mutant for repo⁶⁴. pnt^Δ88 or ttk^B330 have no detectable and minor effects respectively on the neuronal defects seen in embryos expressing gcm^Scer\UAS.cHa under the control of Scer\GAL4^sca-T3.

(Yuasa et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

gcm^Scer\UAS.cHa

gcm^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Hosoya

(Hosoya et al., 1995)

Secondary FlyBase IDs

References (8)

Report Sections

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