FlyBase Allele Report: Dmel\repo[64]

General Information

Symbol

Dmel\repo⁶⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0039835

Feature type

allele

Associated gene

Dmel\repo

Associated Insertion(s)

Carried in Construct

Also Known As

rk2⁶⁴

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Campbell et al., 1994)

Mutagen

ethyl methanesulfonate

(Campbell et al., 1994)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q52term.

(Campbell et al., 1994)

The premature stop codon is before the homeodomain.

(Campbell et al., 1994)

Nucleotide substitution: C301T.

(Campbell et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:18,236,650..18,236,650 [+]

Nucleotide change:

C18236650T

Reported nucleotide change:

C301T

Amino acid change:

Q52term | repo-PA

Reported amino acid change:

Q52term

(Campbell et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | adult stage | dominant

(Matsuno et al., 2015)

abnormal neuroanatomy

(Park et al., 2009)

lethal - all die during embryonic stage | recessive

(Campbell et al., 1994)

some die during embryonic stage | recessive

(Campbell et al., 1994)

Phenotype Manifest In

embryonic longitudinal glial cell

(Park et al., 2009, Campbell et al., 1994)

larval glial cell

(Lee and Jones, 2005)

larval lateral VNC glial cell

(Park et al., 2009)

larval longitudinal connective

(Campbell et al., 1994)

larval ventral nerve cord

(Campbell et al., 1994)

PNS glial cell

(Park et al., 2009)

Detailed Description

Statement

Reference

repo⁶⁴/+ mutants exhibit significantly impaired long term memory performance 24 hours after spaced training in an aversive olfactory association paradigm, as compared to controls; but do not show significantly decreased anesthesia-resistant memory performance 24 hours after massed training in an aversive olfactory association paradigm, as compared to controls.

(Matsuno et al., 2015)

The number of lateral glia is reduced in repo⁶⁴ mutants, while those remaining are disorganised. Most peripheral glia are either missing or displaced and longitudinal glia are displaced toward the lateral edges of the ventral nerve cord.

(Park et al., 2009)

In stage 16 repo⁶⁴ homozygous embryos, the numbers of lateral glia are reduced and those that remain are disorganised, most peripheral glia are either missing or displaced and longitudinal glia are displaced towards the lateral edges of the ventral nerve cord.

(Lee and Jones, 2005)

Gliogenesis and axonogenesis are normal in mutant embryos up to stage 16. At stage 16 longitudinal glial cells are spatially disorganized and reduced in number. By stage 16, mutant embryos have slightly disorganized longitudinal axon fascicles.

(Campbell et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

repo⁶⁴ is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^sca-T3, gcm^UAS.cHa

(Yuasa et al., 2003)

Phenotype Manifest In

Suppressor of

Statement

Reference

repo⁶⁴ is a suppressor of larval neuron phenotype of Scer\GAL4^sca-T3, gcm^UAS.cHa

(Yuasa et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos expressing gcm^Scer\UAS.cHa under the control of Scer\GAL4^sca-T3 show a dramatic restoration of elav-positive cells and neuronal development if they are also mutant for repo⁶⁴.

(Yuasa et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

repo⁶⁴ is partially rescued by repo^UAS.cLa/Scer\GAL4^repo.PL

(Lee and Jones, 2005)

Comments

repo^Scer\UAS.cLa; Scer\GAL4^repo.PL partially rescues the loss and disorganisation of glia seen in repo⁶⁴ homozygous stage 16 embryos. Rescue is most apparent for longitudinal and peripheral glia.

(Lee and Jones, 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

repo⁶⁴

(Matsuno et al., 2015, Park et al., 2009)

repo^rk2

(Lee and Jones, 2005)

rk2⁶⁴

(Yuasa et al., 2003, Campbell et al., 1994)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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