FlyBase Allele Report: Dmel\RpS13[1]

General Information

Symbol

Dmel\RpS13¹

Species

D. melanogaster

Name

FlyBase ID

FBal0048943

Feature type

allele

Associated gene

Dmel\RpS13

Associated Insertion(s)

P{lacW}RpS13¹

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Saeboe-Larssen and Lambertsson, 1996)

Mutagen

P-element activity

(Saeboe-Larssen and Lambertsson, 1996)

Progenitor genotype

Associated Insertion(s)

P{lacW}RpS13¹

Cytology

Description

P{lacW} insertion in the middle of the 5' untranslated leader between nucleotides -15 and -16 relative to the translation initiation codon.

(Saeboe-Larssen and Lambertsson, 1996)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:8,364,578..8,364,578 [-]

(Casad et al., 2011, Oren-Giladi et al., 2008, Saeboe-Larssen and Lambertsson, 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intrinsic cardiomyopathy

CEA

(Casad et al., 2011)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage

(Casad et al., 2011)

increased cell death | larval stage

(Recasens-Alvarez et al., 2021)

lethal | recessive

(Saeboe-Larssen and Lambertsson, 1996)

Minute

(Casad et al., 2011, Marygold et al., 2007)

Minute | dominant

(Coelho et al., 2005)

visible

(Saeboe-Larssen and Lambertsson, 1996)

Phenotype Manifest In

adult heart

(Casad et al., 2011)

imaginal disc | somatic clone

(Recasens-Alvarez et al., 2021)

macrochaeta

(Casad et al., 2011, Saeboe-Larssen and Lambertsson, 1996)

scutellar bristle

(Marygold et al., 2007)

Detailed Description

Statement

Reference

RpS13¹ heterozygous larvae show increased apoptosis in imaginal discs.

(Recasens-Alvarez et al., 2021)

Heterozygous RpS13¹ mutant females exhibit a dilated cardiomyopathy phenotype, including an increased end-systolic dimension (EDD) and a decreased fractional shortening (FS) compared to controls. Bristles appear short and thin compared to wild type (Minute phenotype).

(Casad et al., 2011)

RpS13¹/+ flies have shorter and thinner scutellar bristles.

(Marygold et al., 2007)

Minute phenotype.

(Saeboe-Larssen and Lambertsson, 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

RpS13¹/RpS13[+] is an enhancer of visible phenotype of Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^Bx-MS1096

(Coelho et al., 2005)

Suppressor of

Statement

Reference

RpS13¹/RpS13[+] is a suppressor of increased cell death | somatic clone | larval stage phenotype of Polr1B^k16513

(Grewal et al., 2005)

NOT Suppressor of

Statement

Reference

RpS13¹/RpS13[+] is a non-suppressor of decreased cell number | somatic clone | larval stage phenotype of Polr1B^k16513

(Grewal et al., 2005)

Phenotype Manifest In

Enhancer of

Statement

Reference

RpS13¹/RpS13[+] is an enhancer of wing phenotype of Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^Bx-MS1096

(Coelho et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The proportion of embryos displaying a delay in furrow formation drops from 25% observed for embryos derived from Fmr1³/Df(3R)Exel6265 females to below 25% for embryos from RpS13¹/+;Fmr1³/Df(3R)Exel6265 mothers.

(Monzo et al., 2010)

Homozygous mutant RpI135^k16513 clone survival can be rescued by genetically reducing the growth of surrounding cells by making them heterozygous for a dominant Minute RpS13¹ mutant. In this case, RpI135^k16513 mutant clones are recovered in all RpS13¹ heterozygous discs that are examined at 96hrs after clone induction, a time-point at which all clones are normally eliminated in a wild-type background. However, these clones colonise a significantly smaller area of RpS13¹/+ discs compared with wild-type clones (28.8% compared to 67.8% of the total disc area), indicating that they are still growth impaired. Compared to surrounding heterozygote RpS13¹ or wild-type cells, RpI135^k16513 mutant clone cells exhibit no significant change in either cell size or cell-cycle phasing.

(Grewal et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

2246

y¹ w^*; P{lacW}RpS13¹/CyO

Notes on Origin

Discoverer

Comments

Transcript level and phenotype are restored to wild type by Δ2-3 mobilisation of the P{lacW} insertion demonstrating the mutation is caused by the insertion.

(Saeboe-Larssen and Lambertsson, 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

(Grewal et al., 2005)

M(2)32,P[w⁺]

(Lambertsson et al., 1994)

M(2)32^A1

(Grewal et al., 2005)

M(2)32A¹

RPS13¹

(Recasens-Alvarez et al., 2021)

RpS13¹

(Cassidy et al., 2019, Casad et al., 2011, Monzo et al., 2010, Oren-Giladi et al., 2008, Marygold et al., 2007)

rpS13¹

(Deliu et al., 2022)

Name Synonyms

Minute

(Grewal et al., 2005)

Secondary FlyBase IDs

References (11)

Report Sections

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