Expression of ftz^3-413.hs during embryogenesis using heat shock results in a decrease in the number of wild-type cuticles, with the maximum decrease centered at a time of heat shock of about 2.5 hours after egg laying. The most common phenotype is the "anti-ftz" pair rule phenotype; deletion of ftz-independent parasegments.

Ectopic expression during gastrulation in a ftz^- background results in the anti-ftz phenotype: mutant embryos are deficient for cuticular structures derived from the odd numbered parasegments. An active endogenous ftz gene increases the efficiency of the transformation towards the anti-ftz phenotype.

Deletion of odd numbered parasegments.