FlyBase Allele Report: Ecol\lacZ[FMRFa.WF3]

General Information

Symbol

Ecol\lacZ^FMRFa.WF3

Species

E. coli

Name

FlyBase ID

FBal0049909

Feature type

allele

Associated gene

Ecol\lacZ

Associated Insertion(s)

Carried in Construct

P{WF3}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Schneider et al., 1993)

Progenitor genotype

Ecol\lacZ^C4.Adh

(Schneider et al., 1993)

Carried in construct

P{WF3}

Cytology

Description

8kb fragment of FMRFa from -5000bp to amino acid 23 of exon 2 (containing 5kb upstream sequence, exon I, the entire intron and 69bp of exon II) fused in frame drives the expression of Ecol\lacZ^C4.Adh.

(Schneider et al., 1993)

Allele components

Component

Use(s)

Regulatory region(s)

FMRFa_pWF3

Encoded product / tool

lacZ

reporter enzyme

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 17

apterous-expressing neuron of the lateral cluster of the thorax Ap4 | subset

(Benveniste et al., 1998)

larval stage

thoracic neuron | subset

(Schneider et al., 1993)

larval central nervous system | restricted

(Schneider et al., 1993)

subesophageal nerve | subset

(Schneider et al., 1993)

FMRFamide SE2 neuron

(Schneider et al., 1993)

larval brain | restricted

(Schneider et al., 1993)

apterous-expressing neuron of the lateral cluster of the thorax Ap4 | subset

(Benveniste et al., 1998)

adult stage

adult central nervous system | restricted

(Schneider et al., 1993)

adult ventral nerve cord | restricted

(Schneider et al., 1993)

adult brain | restricted

(Schneider et al., 1993)

adult optic lobe neuron

(Schneider et al., 1993)

Additional Information

Statement

Reference

In 18-20 hour embryos, EcollacZ^Fmrf.WF3 is expressed in ~98% of Tv neurons, and ~96% of first instar larval Tv neurons.

(Benveniste et al., 1998)

In third instar larval CNS, expressed in approximately 60 cells, which fall into 15 distinct cell types. For many of these expression observed in both larva and adult, notably in the Tv and Tva neurons in the thoracic neuromeres; the SE2 interneurons in the subesophageal neuromeres; the OL2 neurons in the optic lobes of the brain. Ectopic expression, not observed for normal FMRFa, is observed in a few cell types, for example, in the X cell neurons.

(Schneider et al., 1993)

Marker for

Reflects expression of

FMRFa

(Benveniste et al., 1998, Schneider et al., 1993)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Ecol\lacZ expression closely resembles the endogenous pattern of Fmrf gene expression.

(Schneider et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ecol\lacZ^FMRFa.WF3

Ecol\lacZ^Fmrf.WF3

lacZ^Fmrf.WF3

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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