FlyBase Recombinant Construct Report: P{WF3}

General Information

Symbol

P{WF3}

FlyBase ID

FBtp0005978

Feature type

transgenic_transposable_element

Component Allele(s)

Ecol\lacZ^FMRFa.WF3

Size

Expression Data

lacZ reporter

Associated insertion(s)

1 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mC

Component allele Ecol\lacZ^FMRFa.WF3

Transgenic product class

Component

Use(s)

Regulatory region(s)

FMRFa_pWF3

Encoded product / tool

lacZ

reporter enzyme

Description

8kb fragment of FMRFa from -5000bp to amino acid 23 of exon 2 (containing 5kb upstream sequence, exon I, the entire intron and 69bp of exon II) fused in frame drives the expression of Ecol\lacZ^C4.Adh.

(Schneider et al., 1993)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Ecol\lacZ^FMRFa.WF3

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 17

apterous-expressing neuron of the lateral cluster of the thorax Ap4 | subset

(Benveniste et al., 1998)

larval stage

thoracic neuron | subset

(Schneider et al., 1993)

larval central nervous system | restricted

(Schneider et al., 1993)

subesophageal nerve | subset

(Schneider et al., 1993)

FMRFamide SE2 neuron

(Schneider et al., 1993)

larval brain | restricted

(Schneider et al., 1993)

apterous-expressing neuron of the lateral cluster of the thorax Ap4 | subset

(Benveniste et al., 1998)

adult stage

adult central nervous system | restricted

(Schneider et al., 1993)

adult ventral nerve cord | restricted

(Schneider et al., 1993)

adult brain | restricted

(Schneider et al., 1993)

adult optic lobe neuron

(Schneider et al., 1993)

Additional Information

Statement

Reference

In 18-20 hour embryos, EcollacZ^Fmrf.WF3 is expressed in ~98% of Tv neurons, and ~96% of first instar larval Tv neurons.

(Benveniste et al., 1998)

In third instar larval CNS, expressed in approximately 60 cells, which fall into 15 distinct cell types. For many of these expression observed in both larva and adult, notably in the Tv and Tva neurons in the thoracic neuromeres; the SE2 interneurons in the subesophageal neuromeres; the OL2 neurons in the optic lobes of the brain. Ectopic expression, not observed for normal FMRFa, is observed in a few cell types, for example, in the X cell neurons.

(Schneider et al., 1993)

Marker for

Reflects expression of

FMRFa

(Benveniste et al., 1998, Schneider et al., 1993)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

FMRFa-LacZ

(Eade et al., 2012)

FMRFa-lacZ

(Allan et al., 2003)

FMRFa-lacZ(WF3-T2)

(Allan et al., 2003)

Fmrf-lacZ#WF3-T2

(Miguel-Aliaga et al., 2004)

P{WF3}

(FlyBase, 1996-)

pWF3

(Taghert et al., 2000)

Secondary FlyBase IDs

References (12)

Report Sections

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