FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Allele: Dmel\grimhs.PC
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General Information
Symbol
Dmel\grimhs.PC
Species
D. melanogaster
Name
heat shock construct of Chen
FlyBase ID
FBal0051531
Feature type
allele
Associated gene
Dmel\grim
Associated Insertion(s)
Carried in Construct
P{HS-grim}
Key Links
Transgenic product class
cDNA
(Chen et al., 1996)
wild_type
(Chen et al., 1996)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Chen et al., 1996)
wild_type
(Chen et al., 1996)
Mutagen
in vitro construct
(Chen et al., 1996)
Progenitor genotype
Carried in construct
P{HS-grim}
Cytology
Description

Expression of an EcoRI fragment of grim cDNA is driven by an Hsp70 promoter.

(Chen et al., 1996)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
grim
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of grimhs.PC results in rapid and robust cell death of 100% of mitotically cycling follicle cells in the ovary. Induction of grimhs.PC during endocycles also results in the death of 100% of follicle cells.

      Expression of grimhs.PC in early third-instar larvae causes rapid apoptosis (caspase activation) in mitotically cycling cells of the wing disc, eye disc and brain. Very weak caspase labelling is seen when grimhs.PC is expressed in endocycling salivary gland cells. No apoptosis is observed in the fat body.

      (Mehrotra et al., 2008)

      Heat induced expression causes extensive cell death throughout the embryo. Ectopic cell death causes gross morphological abnormalities and severe organismal lethality. grim induced apoptosis can occur in Df(3L)H99 embryos.

      (Chen et al., 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      grimhs.PC has increased cell death phenotype, enhanceable by hidhs.PG

      (Mehrotra et al., 2008)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      grimhs.PC has embryonic/larval salivary gland phenotype, enhanceable by hidhs.PG

      (Mehrotra et al., 2008)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of hidhs.PG and grimhs.PC in endocycling salivary gland cells results in a higher level of apoptosis (caspase activation) compared to the expression of either transgene alone. No apoptosis is observed in the fat body.

      (Mehrotra et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      grimhs.PC
      Name Synonyms
      heat shock construct of Chen
      (Chen et al., 1996)
      Secondary FlyBase IDs
        References (3)