Construct: Deletion of 5' flanking sequences, up to -790bp.
Fragment containing the Tm2 coding region, with 790bp 5' flanking sequences, and 2kb 3' flanking sequences. In addition, there is a 10bp BamHI linker inserted at +10bp in the first exon.
100% of Tm23 flies homozygous for Tm2Δ-790 can fly and jump.