FlyBase Allele Report: P1\cre[Mos1.hs]

General Information

Symbol

P1\cre^Mos1.hs

Species

P. Enterobacteria phage P1

Name

FlyBase ID

FBal0057684

Feature type

allele

Associated gene

P1\cre

Associated Insertion(s)

Carried in Construct

P{Crew}

P{Crey}

Also Known As

hs-Cre, P[w⁺, cre], P[w+,cre]

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Siegal and Hartl, 1996)

Progenitor genotype

Carried in construct

P{Crew}

P{Crey}

Cytology

Description

Construct: Fusion of an hsp70 promoter and Dmau\mariner\T^Mos1 promoter (provides translation initiation sequences) drive expression of P1\cre, polyadenylation signal sequence are donated by Dmau\mariner\T^Mos1.

(Siegal and Hartl, 1996)

Allele components

Component

Use(s)

Encoded product / tool

P1\cre

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The genotypic effect of P{Crey} yields an overwhelming majority of white-eyed flies (excision of P{loxP(w^+mC)}), P{Crew} produces yellow eyed progeny (lacks the expression from P{loxP(w^+mC)}, but has weak expression of w^+mC from P{Crew}). 'Paternal' P1\cre transmission effectively represents absence of P1\cre activity, paternal transmission of P{Crey} or P{Crew} yields flies with the eye colour of P{loxP(w^+mC)}. Maternal effect of P{Crey} is not strong, most flies show no sign of P{loxP(w^+mC)} excision, some have sectors of white. Maternal effect of P{Crew} is considerable, most flies only retain small sectors of red.