FlyBase Allele Report: Dmel\okr[17-11]

General Information

Symbol

Dmel\okr^17-11

Species

D. melanogaster

Name

FlyBase ID

FBal0060589

Feature type

allele

Associated gene

Dmel\okr

Associated Insertion(s)

Carried in Construct

Also Known As

A17-11

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Kooistra et al., 1997)

Mutagen

ethyl methanesulfonate

(Kooistra et al., 1997)

Progenitor genotype

Cytology

Description

G to A transition at the splice acceptor site of the second intron.

(Kooistra et al., 1997)

Nucleotide substitution: G?A. Mutation at the splice acceptor site of the second intron.

(Kooistra et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:2,991,279..2,991,279 [-]

Nucleotide change:

G2991279A

Reported nucleotide change:

G?A

Comment:

Mutation at the splice acceptor site of the second intron of okr.

(Kooistra et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye disc | conditional

(Jaklevic and Su, 2004)

metaphase & condensed nuclear chromosome | conditional qualifier

(Jaklevic and Su, 2004)

Detailed Description

Statement

Reference

The survival of okr^17-11/Df(2L)JS17 males carrying a source of transposase (P\T^Δ2-3) and a P-element that can be mobilised (P{}w^hd80617) is reduced to 22% compared to controls lacking a source of transposase, indicating that okr is required for the repair of P-element induced double-strand breaks in males. The survival of okr^17-11/Df(2L)JS17 females carrying a source of transposase (P\T^Δ2-3) and a P-element that can be mobilised (P{}w^hd80617) is reduced to 68% compared to controls lacking a source of transposase.

(Romeijn et al., 2005)

Irradiated mutants regulate both S and M phases and undergo cell death as well as wild-type. However, pupariation is delayed, eye discs degenerate and mutant eye discs are unable to maintain organised cell proliferation. In metaphase chromosomes from larval neuroblasts broken chromosomes are seen in approximately 37% of metaphase cells 3hr after irradiation (compared to about 6% in wild-type).

(Jaklevic and Su, 2004)

Hemizygous okr^17-11/Df(2L)JS17 males larvae in which P-element excision is induced do not survive, and only 10% of females survive. The frequency of reversion of the w^hd80k17 allele (using w^alter as a template) in these flies is reduced fivefold compared to control flies. Hemizygous okr^17-11/Df(2L)JS17 larvae are more sensitive to mitomycin C (MMC) and cis-diamminedichloroplatinum (cisDDP) than control heterozygous larvae. Somatic recombination induced by X rays does not occur in hemizygous flies. Somatic recombination induced by MMC, cisDDP or methyl methanesulfonate is reduced compared to control flies. okr^A19-10/Df(2L)JS17 and okr^A19-10/okr^17-11 mutants have the same methyl methanesulfonate and X ray hypersensitivity as okr^A19-10/Df(2L)JS17 mutants.

(Kooistra et al., 1999)

Early egg development is normal but the eggs do not hatch. The larvae of mutant flies are highly sensitive to X rays and methyl methanesulfonate. The mutants exhibit a defect in induced mitotic recombination after exposure to X-rays.

(Kooistra et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

okr^17-11 has radiation sensitive | embryonic stage phenotype, enhanceable by DNAlig4⁵⁷

(Gorski et al., 2003)

okr^A19-10/okr^17-11 has radiation sensitive phenotype, enhanceable by Blm^D3/Blm^D2

(Kooistra et al., 1999)

Enhancer of

Statement

Reference

okr^17-11/okr^17-11 is an enhancer of radiation sensitive | embryonic stage phenotype of DNAlig4⁵⁷

(Gorski et al., 2003)

okr^A19-10/okr^17-11 is an enhancer of radiation sensitive phenotype of Blm^D3/Blm^D2

(Kooistra et al., 1999)

Other

Statement

Reference

okr^A19-10/okr^17-11, tws⁴³⁰ has lethal - all die before end of first instar larval stage phenotype

(Merigliano et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The presense of Lig4⁵⁷/Y has a greater than additive effect on X-ray induced lethality of okr^17-11/okr^17-11 .

(Gorski et al., 2003)

okr^17-11/okr^A19-10 mus309^D2/mus309^D3 double mutants show a synergistic effect on sensitivity to X rays; the sensitivity is increased 40 fold compared to wild-type in double mutants. okr^17-11/okr^A19-10 mus309^D2/mus309^D3 double mutants do not show a synergistic effect on sensitivity to methyl methanesulfonate.

(Kooistra et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8524

okr^17-11 cn¹ bw¹/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

A17-11

(Romeijn et al., 2005, Jaklevic and Su, 2004)

DmRAD54^17-11

(Eeken et al., 1997)

DmRAD54^A17-11

(Kooistra et al., 1999)

Okra^A17-11

(Romeijn et al., 2005)

Rad54^17-11

Rad54^A17-11

(Gorski et al., 2003)

okr^17-11

(Merigliano et al., 2017)

okr^A17-11

(Cohen et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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