FlyBase Allele Report: Dmel\E(spl)m7-HLH[Act.VP16.UAS]

General Information

Symbol

Dmel\E(spl)m7-HLH^Act.VP16.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0063236

Feature type

allele

Associated gene

Dmel\E(spl)m7-HLH

Associated Insertion(s)

Carried in Construct

P{m7^Act}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Jimenez and Ish-Horowicz, 1997)

Progenitor genotype

Carried in construct

P{m7^Act}

Cytology

Description

UASt regulatory sequences drive expression of the N-terminal 143 amino acids of E(spl)m7-HLH (including the bHLH and adjacent putative helical domains but not the C terminal WRPW tetrapeptide) fused to the VP16 transcriptional activation domain (amino acids 412 to 490).

(Jimenez and Ish-Horowicz, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

E(spl)m7-HLH

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage, with Scer\GAL4^h-1J3

(Jimenez and Ish-Horowicz, 1997)

some die during pupal stage, with Scer\GAL4^h-1J3

(Jimenez and Ish-Horowicz, 1997)

visible, with Scer\GAL4^ptc-559.1

(Jennings et al., 1999)

Phenotype Manifest In

campaniform sensillum of anterior crossvein | ectopic, with Scer\GAL4^ptc-559.1

(Jimenez and Ish-Horowicz, 1997)

dorsal mesothoracic disc & sensory mother cell, with Scer\GAL4^455.2

(Jimenez and Ish-Horowicz, 1997)

leg & macrochaeta, with Scer\GAL4^30A

(Jimenez and Ish-Horowicz, 1997)

macrochaeta | ectopic, with Scer\GAL4^ptc-559.1

(Jennings et al., 1999)

scutum, with Scer\GAL4^ptc-559.1

(Jennings et al., 1999)

scutum & macrochaeta, with Scer\GAL4^h-1J3

(Jimenez and Ish-Horowicz, 1997)

scutum & macrochaeta, with Scer\GAL4^ptc-559.1

(Jennings et al., 1999)

sensory mother cell | ectopic, with Scer\GAL4^455.2

(Jimenez and Ish-Horowicz, 1997)

wing, with Scer\GAL4^ptc-559.1

(Jennings et al., 1999)

wing & macrochaeta, with Scer\GAL4^69B

(Jimenez and Ish-Horowicz, 1997)

wing & macrochaeta, with Scer\GAL4^ptc-559.1

(Jennings et al., 1999)

wing & sensory mother cell, with Scer\GAL4^455.2

(Jimenez and Ish-Horowicz, 1997)

wing vein | ectopic, with Scer\GAL4^69B

(Jimenez and Ish-Horowicz, 1997)

Detailed Description

Statement

Reference

Expression of HLHm7^{Act.Scer\UAS.T:Hsim\VP16} under the control of Scer\GAL4^ptc-559.1 gives rise to ectopic bristles along the anteroposterior boundary of the wing and on the notum.

(Jennings et al., 1999)

Scer\GAL4^h-1J3-mediated expression causes the development of extra bristles on the notum (dramatic transformation of cuticle into bristles), pupae are dissected out of the pupal case. Scer\GAL4^30A-mediated expression causes the development of extra bristles on the leg. Scer\GAL4^69B-mediated expression causes the development of extra bristles on the wing as well as ectopic wing vein tissue. Scer\GAL4^ptc-559.1-mediated expression induces ectopic campaniform sensilla at the anterior crossvein. Scer\GAL4^455.2-mediated expression induces ectopic sensory organ mother cells in the presumptive scutellum region of the wing disc.

(Jimenez and Ish-Horowicz, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of HLHm7^{Act.Scer\UAS.T:Hsim\VP16} under the control of Scer\GAL4^pnr-MD237 induces bristle formation, even in a Df(1)sc10-1/Y background.

(Giagtzoglou et al., 2003)

Scer\GAL4^455.2-mediated expression in the absence of ac and sc (Df(1)sc10-1) allows no development of ectopic SMCs and bristles, lack of ac (In(1)y^3PLsc^8R) or sc (sc^M6) reduces the number of ectopic bristles. Deletion of sc promoter and coding sequence (In(1)sc^8Lsc^4R) does not suppress the formation of ectopic bristles.

(Jimenez and Ish-Horowicz, 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51671

w^*; P{m7^Act}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

E(spl)m7-HLH^{Act.Scer\UAS.T:Hsim\VP16}

E(spl)m7-HLH^Act.VP16.UAS

HLHm7::VP16^Act.UAS

HLHm7^{Act.Scer\UAS.T:Hsim\VP16}

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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