An Hsp70 promoter drives expression of a mutated form of Tn9CAT in which codon 33 has been mutated from a Tyr residue to an amber stop codon (codon is changed from TAT to TAG).
tRNA:Y1:22FaDtT.Su+ can suppress the amber mutation of Ecol\CATY33stop.hs, but the level of suppression is low.
tRNA:Y1:22FaDtT.Su+ can suppress the amber mutation of Ecol\CATY33stop.hs, but the level of suppression is low.