FlyBase Allele Report: Dmel\αTub85E[βTub85D.PH]

General Information

Symbol

Dmel\αTub85E^βTub85D.PH

Species

D. melanogaster

Name

β-Tubulin at 85D promoter construct of Hutchens

FlyBase ID

FBal0066288

Feature type

allele

Associated gene

Dmel\αTub85E

Associated Insertion(s)

Carried in Construct

P{α85E}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Hutchens et al., 1997)

Progenitor genotype

Carried in construct

P{α85E}

Cytology

Description

1860bp DraI-HincII fragment of genomic αTub85E DNA is cloned into the male germ line expression vector containing βTub85D 5' and 3' sequences.

(Hutchens et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

βTub85D

Encoded product / tool

αTub85E

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male fertile

(Hoyle et al., 2001)

Phenotype Manifest In

spermatid

(Hutchens et al., 1997)

Detailed Description

Statement

Reference

Males carrying one copy of αTub85E^βTub85D.PH in a wild-type background are fertile.

(Hoyle et al., 2001)

Four copies causes some spermatids to be misaligned, a lower copy number does not affect spermatid alignment. The ultrastructure of the axoneme is defective: expression causes defects in morphogenesis of the central pair and accessory microtubules. Expression does not affect the spindles or other classes of cytoplasmic microtubules.

(Hutchens et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

αTub85E^βTub85D.PH, βTub85D⁴³² has male sterile phenotype

(Hoyle et al., 2001)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Males carrying one copy of αTub85E^βTub85D.PH and one copy of βTub85D⁴³² in an otherwise wild-type background are sterile.

(Hoyle et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Transheteroallelic combinations of αTub84B produces males that are sterile or weakly fertile. Expression of αTub85E^βTub85D.PH partially rescues the defective αTub84B function in spermatogenesis: most spermatids have complete axonemes.

(Hutchens et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

αTub85E^βTub85D.PH

βTub85D^βTub60D.PHa

Name Synonyms

β-Tubulin at 85D promoter construct of Hutchens

(Hutchens et al., 1997)

Secondary FlyBase IDs

References (2)

Report Sections

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