FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Rab1N124I.UAS
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General Information
Symbol
Dmel\Rab1N124I.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0083266
Feature type
allele
Associated gene
Dmel\Rab1
Associated Insertion(s)
Carried in Construct
P{UAS-Rab1.N124I}
Key Links
Transgenic product class
dominant_negative_variant
(Satoh et al., 1997)
missense_variant
(Satoh et al., 1997)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Satoh et al., 1997)
missense_variant
(Satoh et al., 1997)
Mutagen
in vitro construct
(Satoh et al., 1997)
Progenitor genotype
Carried in construct
P{UAS-Rab1.N124I}
Cytology
Description

UASt regulatory sequences drive expression of a dominant-negative form of Rab1. The protein contains the amino acid replacement N124I within the third conserved region for guanine nucleotide binding.

(Satoh et al., 1997)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rab1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      Golgi apparatus & photoreceptor cell, with Scer\GAL4hs.PB

      (Satoh et al., 1997)

      microvillus & photoreceptor cell, with Scer\GAL4hs.PB

      (Satoh et al., 1997)

      rough endoplasmic reticulum & photoreceptor cell, with Scer\GAL4hs.PB

      (Satoh et al., 1997)
      Detailed Description
      Statement
      Reference

      Scer\GAL4hs.PB-mediated expression causes the lumens of rough ER to become swollen and Golgi bodies are disassembled into vesicle clusters. Rhodopsin transport between the rER and Golgi body is blocked. Long term expression causes the degradation of photoreceptor microvilli and the accumulation of numerous swollen rERs.

      (Satoh et al., 1997)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Dominant negative protein.

      (Satoh et al., 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Rab1N124I.Scer\UAS
      Rab1N124I.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)