Generation of Hr46^R107G mutant clones result in a high frequency of adults (62%) with wings that are curved or folded and held out from the body, with the position of the clones corresponding to the deformations seen in the wing. Legs appear wild-type in phenotype. No reduction in size of clones is seen. When clones that cover at least a third of the notum are made in a minute background, some of these clones have cuticle that is lighter in colour, apparently immature, some display indentations in the notum, and short and rough bristles are also seen. Defects in the abdomen are also seen. In small clones of 1 to 2 bristles in size, bristles of apparently normal morphology sometimes point laterally instead of to the posterior as normal. Also clones at the segmental boundaries display an absence of black pigmentation. Larger clones show distortions in the structure of the abdomen, lost pigmentation and missing bristles. Loss of Hr46 activity is not cell lethal.

Hemizygotes generally die during the embryonic stage. Hemizygous embryos lack lateral cluster neurons as well as most ventral cluster neurons in the peripheral nervous system. 14% of these embryos have a break in the posterior portion of the longitudinal tract of the central nervous system.