Reporter gene expression is observed in leg imaginal discs isolated from late third instar larvae. Expression is initially restricted to the precursors of the tarsal segments. In prepupal leg discs, the expression expands to the precursors of the tibia and the femur. Expression is induced in the salivary glands at puparium formation. Expression is also observed in the fat body and in the trachea. Expression is observed in the ventral ganglion and in the presumptive optic lobes of the CNS in mid-third instar larvae and increases noticably at puparium formation. Finally, expression is observed in the corpus allatum of the ring gland.

In early embryo, expression in an 'anterior ring of cells'; expression in mandibular segment not certain.

In wandering third instar larva, no detectable β-gal staining.