FlyBase Allele Report: Dmel\Ras85D[V12.G37.UAS]

General Information

Symbol

Dmel\Ras85D^V12.G37.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0085937

Feature type

allele

Associated gene

Dmel\Ras85D

Associated Insertion(s)

Carried in Construct

P{UAS-Ras85D.V12.G37}

Also Known As

UAS-Ras^V12G37

Key Links

Transgenic product class

missense_variant

(Karim and Rubin, 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Karim and Rubin, 1998)

Mutagen

in vitro construct

(Karim and Rubin, 1998)

Progenitor genotype

Ras85D^V12.UAS

(Karim and Rubin, 1998)

Carried in construct

P{UAS-Ras85D.V12.G37}

Cytology

Description

A mutation has been introduced into the effector loop (amino acid replacement E37G).

(Karim and Rubin, 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ras85D

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^dpp.blk1

(Karim and Rubin, 1998)

eye, with Scer\GAL4^hs.2sev

(Therrien et al., 1999)

mitotic cell cycle | somatic clone, with Scer\GAL4^Act5C.PP

(Prober and Edgar, 2002)

ommatidium, with Scer\GAL4^{E(spl)mδ-HLH.0.5}

(Gaengel and Mlodzik, 2003)

ommatidium, with Scer\GAL4^hs.2sev

(Gaengel and Mlodzik, 2003)

photoreceptor neuron | P-stage | ectopic, with Scer\GAL4^GMR.PF

(Yang and Baker, 2003)

type I bouton | increased number, with Scer\GAL4^futsch-C380

(Koh et al., 2002)

wing disc, with Scer\GAL4^dpp.blk1

(Karim and Rubin, 1998)

wing vein, with Scer\GAL4^dpp.blk1

(Karim and Rubin, 1998)

Detailed Description

Statement

Reference

Fly hearts expressing Scer\GAL4^tin.CΔ4>Ras85D^{V12.G37.Scer\UAS} show end-diastolic dimensions similar to wild-type. Histological analysis of cardiac chambers from adult transgenic flies show heart wall thickness that is similar to that of controls. The number of cardiomyocytes in the transgenic hearts is also similar to that of wild-type.

(Yu et al., 2013)

Expression of Ras85D^{V12.S35.Scer\UAS} in the midgut, under the control of Scer\GAL4^esg-NP7397 does not induce midgut cell proliferation.

(Jiang et al., 2011)

Ectopic expression of Ras85D^{V12.G37.Scer\UAS} driven by Scer\GAL4^esg-NP7397 in adult midgut progenitor cells during larval development has little effect on their proliferation.

(Jiang and Edgar, 2009)

Expression of Ras85D^{V12.G37.Scer\UAS} under the control of Scer\GAL4^ey.PH early in the entire eye does not cause any tissue overgrowth or increase in organ size.

(Yan et al., 2009)

Ras85D^{V12.G37.Scer\UAS}; Scer\GAL4^elav-C155 animals are viable, and do not exhibit brain degeneration.

(Botella et al., 2003)

When Ras85D^{V12.G37.Scer\UAS} is driven by Scer\GAL4^HLHmδ ommatidial misrotations are seen in the eye. When Ras85D^{V12.G37.Scer\UAS} is driven by Scer\GAL4^hs.2sev ommatidial misrotations are seen in the eye.

(Gaengel and Mlodzik, 2003)

The distribution and morphology of macrochaetae and microchaetae is normal in animals expressing Ras85D^{V12.G37.Scer\UAS} under the control of Scer\GAL4^sca.PU.

Expression of Ras85D^{V12.G37.Scer\UAS} under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype.

(Mirey et al., 2003)

Caspase activation in cells posterior to the furrow in eye discs is completely suppressed by Ras85D^{V12.G37.Scer\UAS}; Scer\GAL4^GMR.PF. There is no accompanying increase in the levels of photoreceptor differentiation at this stage, but there is an increase in mitosis during the second mitotic wave (G2 to M progression, posterior to the furrow). The number of cells displaying caspase activation in pupal eye discs at 30 hours after puparium formation, is also significantly reduced in these animals, accompanied by significantly increased photoreceptor differentiation.

(Yang and Baker, 2003)

Expression of Ras85D^{V12.G37.Scer\UAS}, under the control of Scer\GAL4^C380 increases the number of type I synaptic boutons compared to wild-type controls. This increase is indistinguishable from the increase in bouton number observed in flies where Ras85D^Scer\UAS.cKa expression is driven by Scer\GAL4^C380.

(Koh et al., 2002)

Expression of Ras85D^{V12.G37.Scer\UAS} under the control of Scer\GAL4^Act5C.PP in wing disc cells results in an increase in cell size and an increase in clone area compared to controls. The proportion of cells in G₁ is decreased compared to controls. Clones expressing Ras85D^{V12.G37.Scer\UAS} under the control of Scer\GAL4^Act5C.PP are not visibly round, do not generate ectopic folds and do not bulge out of the epithelium.

(Prober and Edgar, 2002)

When expression is driven by Scer\GAL4^hs.2sev a rough eye phenotype results.

(Therrien et al., 1999)

Scer\GAL4^dpp.blk1-mediated expression causes little, if any, hyperplastic growth in third instar wing discs. Mutants survive to adulthood and have minor differentiation defects (mild rough eyes and extra wing vein material).

(Karim and Rubin, 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ras85D^V12.G37.UAS, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Rgl^unspecified/Rgl^unspecified

(Mirey et al., 2003)

Ras85D^V12.G37.UAS, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Rala^S25N.cMa.UAS, Scer\GAL4^GMR.PU

(Mirey et al., 2003)

NOT Enhanced by

Statement

Reference

Ras85D^V12.G37.UAS, Scer\GAL4^GMR.PU has visible phenotype, non-enhanceable by Rgl^1FL.UAS, Scer\GAL4^GMR.PU

(Mirey et al., 2003)

Suppressed by

Statement

Reference

Rala^S25N.cMa.UAS, Ras85D^V12.G37.UAS, Scer\GAL4^sca.PU has visible phenotype, suppressible by Rgl^{1.UAS.Tag:PM(hKRAS)}, Scer\GAL4^sca.PU

(Mirey et al., 2003)

NOT suppressed by

Statement

Reference

Ras85D^V12.G37.UAS, Scer\GAL4^GMR.PU has visible phenotype, non-suppressible by Rgl^1FL.UAS, Scer\GAL4^GMR.PU

(Mirey et al., 2003)

Enhancer of

Statement

Reference

Ras85D^V12.G37.UAS, Scer\GAL4^sca.PU is an enhancer of visible phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ras85D^V12.G37.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Rgl^unspecified/Rgl^unspecified

(Mirey et al., 2003)

Ras85D^V12.G37.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Rala^S25N.cMa.UAS, Scer\GAL4^GMR.PU

(Mirey et al., 2003)

Ras85D^V12.G37.UAS, Scer\GAL4^hs.2sev has eye phenotype, enhanceable by cnk^{Nterm.UAS.Tag:FLAG}, Scer\GAL4^hs.2sev

(Therrien et al., 1999)

Ras85D^V12.G37.UAS, Scer\GAL4^hs.2sev has eye phenotype, enhanceable by cnk^{PDZmut.UAS.Tag:FLAG}, Scer\GAL4^hs.2sev

(Therrien et al., 1999)

Ras85D^V12.G37.UAS, Scer\GAL4^hs.2sev has eye phenotype, enhanceable by cnk^{hs.sev.Tag:FLAG}

(Therrien et al., 1999)

Ras85D^V12.G37.UAS, Scer\GAL4^hs.2sev has eye phenotype, enhanceable by cnk^{SAMmut.hs.sev.Tag:FLAG}

(Therrien et al., 1999)

NOT Enhanced by

Statement

Reference

Ras85D^V12.G37.UAS, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Rgl^1FL.UAS, Scer\GAL4^GMR.PU

(Mirey et al., 2003)

Ras85D^V12.G37.UAS, Scer\GAL4^hs.2sev has eye phenotype, non-enhanceable by cnk^{CRICmut.UAS.Tag:FLAG}, Scer\GAL4^hs.2sev

(Therrien et al., 1999)

Suppressed by

Statement

Reference

Rala^S25N.cMa.UAS, Ras85D^V12.G37.UAS, Scer\GAL4^sca.PU has chaeta phenotype, suppressible by Rgl^{1.UAS.Tag:PM(hKRAS)}, Scer\GAL4^sca.PU

(Mirey et al., 2003)

NOT suppressed by

Statement

Reference

Ras85D^V12.G37.UAS, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by Rgl^1FL.UAS, Scer\GAL4^GMR.PU

(Mirey et al., 2003)

Enhancer of

Statement

Reference

Ras85D^V12.G37.UAS, Scer\GAL4^sca.PU is an enhancer of microchaeta phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Ras85D^V12.G37.UAS, Scer\GAL4^sca.PU is an enhancer of macrochaeta phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

NOT Suppressor of

Statement

Reference

Ras85D^V12.G37.UAS/Scer\GAL4^hs.2sev is a non-suppressor of phenotype of hid^GMR.PG

(Bergmann et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Ras85D^{V12.G37.Scer\UAS} dramatically enhances the loss of bristle phenotype caused by expression of Rala^{S25N.cMa.Scer\UAS} under the control of Scer\GAL4^sca.PU.

The loss of bristle phenotype seen in flies co-expressing Ras85D^{V12.G37.Scer\UAS} and Rala^{S25N.cMa.Scer\UAS} under the control of Scer\GAL4^sca.PU is largely suppressed by co-expression of Rgl^{1.Scer\UAS.T:Hsap\CAAX}.

Co-expression of Rala^{S25N.cMa.Scer\UAS} enhances the rough eye phenotype caused by expression of Ras85D^{V12.G37.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

The rough eye phenotype caused by expression of Ras85D^{V12.G37.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced in Rgl^unspecified homozygotes.

Co-expression of Rgl^1FL.Scer\UAS has no effect on the rough eye phenotype caused by expression of Ras85D^{V12.G37.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

(Mirey et al., 2003)

The enhancement effect of Ras85D^{V12.G37.Scer\UAS} on cnk^{hs.sev.T:Zzzz\FLAG} contrasts sharply with the lack of enhancement of cnk^{hs.sev.T:Zzzz\FLAG} by Ras85D^{V12.S35.Scer\UAS} or Ras85D^{V12.C40.Scer\UAS}. When Ras85D^{V12.G37.Scer\UAS} is expressed from Scer\GAL4^hs.2sev in combination with cnk^{Nterm.Scer\UAS.T:Zzzz\FLAG} or cnk^{PDZmut.Scer\UAS.T:Zzzz\FLAG} the enhancement to the roughening phenotype is extreme, whereas when expressed in combination with cnk^{SAMmut.hs.sev.T:Zzzz\FLAG} the roughening is only slightly enhanced.

(Therrien et al., 1999)

When expression is driven by Scer\GAL4^hs.2sev, has no effect on the W^GMR.PG eye phenotype.

(Bergmann et al., 1998)

Ras85D^{V12.G37.Scer\UAS} Scer\GAL4^dpp.blk1 and rl^Sem act synergistically to produce a small amount of hyperplastic growth in third instar wing discs. Mutants die as pharate adults at 25^oC and have deletions of tarsal segments and extra wing vein material along L3.

(Karim and Rubin, 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Ras1V12G37

(Koh et al., 2002)

Ras85D^{V12.G37.Scer\UAS}

Ras85D^V12.G37.UAS

Ras85D^V12G37

(Gaengel and Mlodzik, 2003)

Name Synonyms

Secondary FlyBase IDs

References (15)

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