FlyBase Allele Report: Dmel\Ras85D[V12.C40.UAS]

General Information

Symbol

Dmel\Ras85D^V12.C40.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0085938

Feature type

allele

Associated gene

Dmel\Ras85D

Associated Insertion(s)

Carried in Construct

P{UAS-Ras85D.V12.C40}

Key Links

Transgenic product class

missense_variant

(Karim and Rubin, 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Karim and Rubin, 1998)

Mutagen

in vitro construct

(Karim and Rubin, 1998)

Progenitor genotype

Ras85D^V12.UAS

(Karim and Rubin, 1998)

Carried in construct

P{UAS-Ras85D.V12.C40}

Cytology

Description

A mutation has been introduced into the effector loop (amino acid replacement Y40C).

(Karim and Rubin, 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ras85D

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity, with Scer\GAL4^{E(spl)mδ-HLH.0.5}

(Gaengel and Mlodzik, 2003)

abnormal cell polarity, with Scer\GAL4^hs.2sev

(Gaengel and Mlodzik, 2003)

abnormal neuroanatomy, with Scer\GAL4^futsch-C380

(Koh et al., 2002)

hyperplasia, with Scer\GAL4^dpp.blk1

(Karim and Rubin, 1998)

viable, with Scer\GAL4^elav-C155

(Botella et al., 2003)

visible, with Scer\GAL4^dpp.blk1

(Karim and Rubin, 1998)

visible, with Scer\GAL4^hs.2sev

(Therrien et al., 1999)

Phenotype Manifest In

bouton | increased number, with Scer\GAL4^futsch-C380

(Koh et al., 2002)

eye, with Scer\GAL4^hs.2sev

(Therrien et al., 1999)

eye photoreceptor cell, with Scer\GAL4^sev.PU

(Bigenzahn et al., 2018)

ommatidium, with Scer\GAL4^{E(spl)mδ-HLH.0.5}

(Gaengel and Mlodzik, 2003)

ommatidium, with Scer\GAL4^hs.2sev

(Gaengel and Mlodzik, 2003)

ommatidium, with Scer\GAL4^sev.PU

(Bigenzahn et al., 2018)

wing, with Scer\GAL4^dpp.blk1

(Karim and Rubin, 1998)

wing disc, with Scer\GAL4^dpp.blk1

(Karim and Rubin, 1998)

wing vein, with Scer\GAL4^dpp.blk1

(Karim and Rubin, 1998)

Detailed Description

Statement

Reference

Expression of Ras85D^V12.C40.UAS under the control of Scer\GAL4^sev.PU leads to ommatidial rotation defects and loss of the R7 or other photoreceptor cells when compared to controls.

(Bigenzahn et al., 2018)

Expression of Ras85D^{V12.C40.Scer\UAS} under the control of Scer\GAL4^ey.PH early in the entire eye does not cause any tissue overgrowth or increase in organ size.

(Yan et al., 2009)

Ras85D^{V12.C40.Scer\UAS}; Scer\GAL4^elav-C155 animals are viable, and do not exhibit brain degeneration.

(Botella et al., 2003)

When Ras85D^{V12.C40.Scer\UAS} is driven by Scer\GAL4^HLHmδ some weak ommatidial misrotations are seen in the eye. When Ras85D^V12.Scer\UAS is driven by Scer\GAL4^hs.2sev ommatidial misrotations are seen in the eye.

(Gaengel and Mlodzik, 2003)

The distribution and morphology of macrochaetae and microchaetae is normal in animals expressing Ras85D^{V12.C40.Scer\UAS} under the control of Scer\GAL4^sca.PU.

Expression of Ras85D^{V12.C40.Scer\UAS} under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype.

(Mirey et al., 2003)

Expression of Ras85D^{V12.C40.Scer\UAS}, under the control of Scer\GAL4^C380 increases the number of type I synaptic boutons compared to wild-type controls. This increase is indistinguishable from the increase in bouton number observed in flies where Ras85D^Scer\UAS.cKa expression is driven by Scer\GAL4^C380.

(Koh et al., 2002)

Expression of Ras85D^{V12.C40.Scer\UAS} under the control of Scer\GAL4^Act5C.PP in wing disc cells does not result in a significant increase in clone area relative to controls.

(Prober and Edgar, 2002)

When expression is driven by Scer\GAL4^hs.2sev a rough eye phenotype results.

(Therrien et al., 1999)

No ectopic proliferation is seen with Ras85D^{V12.C40.Scer\UAS}. The adults often have wing blisters and extra vein material along L3.

(Karim and Rubin, 1998)

Scer\GAL4^dpp.blk1-mediated expression causes little, if any, hyperplastic growth in third instar wing discs. Mutants survive to adulthood and have minor differentiation defects.

(Karim and Rubin, 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Ras85D^V12.C40.UAS, Scer\GAL4^GMR.PU has visible phenotype, non-enhanceable by Rala^S25N.cMa.UAS, Scer\GAL4^GMR.PU

(Mirey et al., 2003)

Ras85D^V12.C40.UAS, Scer\GAL4^GMR.PU has visible phenotype, non-enhanceable by Rgl^1FL.UAS, Scer\GAL4^GMR.PU

(Mirey et al., 2003)

Ras85D^V12.C40.UAS, Scer\GAL4^hs.2sev has visible phenotype, non-enhanceable by cnk^{hs.sev.Tag:FLAG}

(Therrien et al., 1999)

Suppressed by

Statement

Reference

Rala^S25N.cMa.UAS, Ras85D^V12.C40.UAS, Scer\GAL4^sca.PU has visible phenotype, suppressible by Rgl^{1.UAS.Tag:PM(hKRAS)}, Scer\GAL4^sca.PU

(Mirey et al., 2003)

NOT suppressed by

Statement

Reference

Ras85D^V12.C40.UAS, Scer\GAL4^GMR.PU has visible phenotype, non-suppressible by Rala^S25N.cMa.UAS, Scer\GAL4^GMR.PU

(Mirey et al., 2003)

Ras85D^V12.C40.UAS, Scer\GAL4^GMR.PU has visible phenotype, non-suppressible by Rgl^1FL.UAS, Scer\GAL4^GMR.PU

(Mirey et al., 2003)

Enhancer of

Statement

Reference

Ras85D^V12.C40.UAS, Scer\GAL4^sca.PU is an enhancer of visible phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Other

Statement

Reference

Ras85D^V12.C40.UAS, Scer\GAL4^Appl.G1a, vap² has lethal phenotype

(Botella et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ras85D^V12.C40.UAS, Scer\GAL4^sev.PU has ommatidium phenotype, enhanceable by Lztr1^GD4892, Scer\GAL4^sev.PU

(Bigenzahn et al., 2018)

NOT Enhanced by

Statement

Reference

Ras85D^V12.C40.UAS, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Rala^S25N.cMa.UAS, Scer\GAL4^GMR.PU

(Mirey et al., 2003)

Ras85D^V12.C40.UAS, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Rgl^1FL.UAS, Scer\GAL4^GMR.PU

(Mirey et al., 2003)

Suppressed by

Statement

Reference

Ras85D^V12.C40.UAS, Scer\GAL4^sev.PU has eye photoreceptor cell phenotype, suppressible | partially by Lztr1^GD4339, Scer\GAL4^sev.PU

(Bigenzahn et al., 2018)

Ras85D^V12.C40.UAS, Scer\GAL4^sev.PU has eye photoreceptor cell phenotype, suppressible | partially by Lztr1^GD4892, Scer\GAL4^sev.PU

(Bigenzahn et al., 2018)

Ras85D^V12.C40.UAS, Scer\GAL4^sev.PU has eye photoreceptor cell phenotype, suppressible | partially by Lztr1^HMS00306, Scer\GAL4^sev.PU

(Bigenzahn et al., 2018)

Rala^S25N.cMa.UAS, Ras85D^V12.C40.UAS, Scer\GAL4^sca.PU has chaeta phenotype, suppressible by Rgl^{1.UAS.Tag:PM(hKRAS)}, Scer\GAL4^sca.PU

(Mirey et al., 2003)

NOT suppressed by

Statement

Reference

Ras85D^V12.C40.UAS, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by Rala^S25N.cMa.UAS, Scer\GAL4^GMR.PU

(Mirey et al., 2003)

Ras85D^V12.C40.UAS, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by Rgl^1FL.UAS, Scer\GAL4^GMR.PU

(Mirey et al., 2003)

Enhancer of

Statement

Reference

Ras85D^V12.C40.UAS, Scer\GAL4^sca.PU is an enhancer of microchaeta phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Ras85D^V12.C40.UAS, Scer\GAL4^sca.PU is an enhancer of macrochaeta phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Suppressor of

Statement

Reference

Ras85D^V12.C40.UAS/Scer\GAL4^hs.2sev is a suppressor of ommatidium phenotype of hid^GMR.PG

(Bergmann et al., 1998)

Ras85D^V12.C40.UAS/Scer\GAL4^hs.2sev is a suppressor of eye phenotype of hid^GMR.PG

(Bergmann et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Ras85D^{V12.C40.Scer\UAS} dramatically enhances the loss of bristle phenotype caused by expression of Rala^{S25N.cMa.Scer\UAS} under the control of Scer\GAL4^sca.PU.

The loss of bristle phenotype seen in flies co-expressing Ras85D^{V12.C40.Scer\UAS} and Rala^{S25N.cMa.Scer\UAS} under the control of Scer\GAL4^sca.PU is suppressed by co-expression of Rgl^{1.Scer\UAS.T:Hsap\CAAX}.

Co-expression of either Rala^{S25N.cMa.Scer\UAS} or Rgl^1FL.Scer\UAS has no effect on the rough eye phenotype caused by expression of Ras85D^{V12.C40.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

(Mirey et al., 2003)

The enhancement effect of Ras85D^{V12.G37.Scer\UAS} on cnk^{hs.sev.T:Zzzz\FLAG} contrasts sharply with the lack of enhancement of cnk^{hs.sev.T:Zzzz\FLAG} by Ras85D^{V12.S35.Scer\UAS} or Ras85D^{V12.C40.Scer\UAS}.

(Therrien et al., 1999)

When expression is driven by Scer\GAL4^hs.2sev, acts as a moderate suppressor of the W^GMR.PG eye phenotype.

(Bergmann et al., 1998)

Ras85D^{V12.C40.Scer\UAS} Scer\GAL4^dpp.blk1 and rl^Sem do not act synergistically, no hyperplastic growth is seen in third instar wing discs. The adults often have wing blisters and extra vein material along L3. This phenotype is not suppressed by phl^XS-2541.

(Karim and Rubin, 1998)

No ectopic proliferation is seen with Ras85D^{V12.C40.Scer\UAS}. The adults often have wing blisters and extra vein material along L3. This phenotype is not suppressed by Raf^XS-2541.

(Karim and Rubin, 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Ras1V12C40

(Koh et al., 2002)

Ras85D^{V12.C40.Scer\UAS}

Ras85D^V12.C40.UAS

Ras85D^V12C40

(Gaengel and Mlodzik, 2003)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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