FlyBase Allele Report: Dmel\eIF4E3[L0139]

General Information

Symbol

Dmel\eIF4E3^L0139

Species

D. melanogaster

Name

FlyBase ID

FBal0086986

Feature type

allele

Associated gene

Dmel\eIF4E3

Associated Insertion(s)

P{lacW}eIF4E3^L0139

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Hernandez et al., 2012)

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}eIF4E3^L0139

Cytology

Description

Insertion 14bp upstream of the 5' end of the predicted transcription start site.

(Hernandez et al., 2012)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:8,229,237..8,229,237 [+]

(Hernandez et al., 2012, Spradling et al., 1999, Nicholls and Gelbart, 1998, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | male (with Df(3L)BSC732)

(Hernandez et al., 2012)

female fertile (with Df(3L)BSC732)

(Hernandez et al., 2012)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

male sterile (with Df(3L)BSC376)

(Hernandez et al., 2012)

male sterile (with Df(3L)BSC732)

(Hernandez et al., 2012)

male sterile (with Df(3L)ED4408)

(Hernandez et al., 2012)

male sterile (with Df(3L)Exel6112)

(Hernandez et al., 2012)

Phenotype Manifest In

elongation stage spermatid (with Df(3L)BSC732)

(Hernandez et al., 2012)

Nebenkern derivative (with Df(3L)BSC732)

(Hernandez et al., 2012)

pre-elongation spermatid (with Df(3L)BSC732)

(Hernandez et al., 2012)

seminal vesicle (with Df(3L)BSC732)

(Hernandez et al., 2012)

spermatozoon (with Df(3L)BSC732)

(Hernandez et al., 2012)

sperm individualization complex (with Df(3L)BSC732)

(Hernandez et al., 2012)

Detailed Description

Statement

Reference

eIF4E-3^L0139/Df(3L)BSC732 spermatids show defects in meiotic cytokinesis and chromosome segregation. Early round spermatids contain multiple nuclei accompanied by enlarged mitochondrial derivatives. Many of these multinucleate spermatids have micronuclei. Elongated spermatid cysts are formed, but they show defects in nuclear shaping, with the nuclei remaining relatively round. The individualisation complex does not form, resulting in the complete disruption of individualisation and the absence of mature sperm. The seminal vesicles are consistently smaller and the basal region less dense than in wild-type testes.

(Hernandez et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

10169

y¹ w¹¹¹⁸; P{lacW}eIF4E3^L0139/TM3, Ser¹

Notes on Origin

Discoverer

Comments

Complements: l(3)L4111^L4111.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

eIF4E-3^L0139

(Hernandez et al., 2012)

eIF4E3^L0139

l(3)L0139

(Spradling et al., 1999)

l(3)L0139^L0139

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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