FlyBase Allele Report: Dmel\smid[C161]

General Information

Symbol

Dmel\smid^C161

Species

D. melanogaster

Name

FlyBase ID

FBal0088633

Feature type

allele

Associated gene

Dmel\smid

Associated Insertion(s)

P{GawB}smid^C161

Carried in Construct

Also Known As

C161-GAL4, c161, smid-GAL4

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Long et al., 1998, Long et al., 1998)

Mutagen

P-element activity

(Long et al., 1998)

Progenitor genotype

Associated Insertion(s)

P{GawB}smid^C161

Cytology

Description

P{GawB} insertion close to the transcriptional start site, within the 5' untranslated region of the transcription unit.

(Long et al., 1998)

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm | recessive

(Long et al., 1998)

decreased occurrence of cell division | larval stage

(Long et al., 1998)

increased cell size | larval stage

(Long et al., 1998)

lethal - all die before end of larval stage | recessive

(Long et al., 1998)

some die during larval stage | recessive

(Long et al., 1998)

Phenotype Manifest In

central nervous system

(Long et al., 1998)

larval brain

(Long et al., 1998)

larval central nervous system

(Long et al., 1998)

neuroblast | larval stage

(Long et al., 1998)

Detailed Description

Statement

Reference

Larvae exhibit poor locomotion and become developmentally arrested as second instar larvae, they survive as second instar for up to 8 weeks without further development before dying. Larvae exhibit considerably reduced central nervous system.

(Long et al., 1998)

Homozygous larvae usually show no detectable BrdU incorporation in the central nervous system, and in all cases the number of neuroblasts incorporating BrdU is significantly less than in wild-type. The neuroblasts enlarge normally but fail to begin mitosis. The pattern of cell death revealed by TUNEL staining in larval brains is different from wild-type larvae.

(Long et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27893

w^*; P{GawB}smid^C161/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Mobilisation of the P{GawB} insertion demonstrates it is responsible for the lethality.

(Long et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

smid¹

(Long et al., 1998)

smid^C161

(Cook and Cook, 2010.5.7)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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