FlyBase Allele Report: Dmel\Sema2a[UAS.cKa]

General Information

Symbol

Dmel\Sema2a^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kolodkin

FlyBase ID

FBal0090137

Feature type

allele

Associated gene

Dmel\Sema2a

Associated Insertion(s)

Carried in Construct

P{UAS-sema-II.K}

Also Known As

UAS-Sema-2a, UAS-sema2a

Key Links

Transgenic product class

wild_type

(Winberg et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Winberg et al., 1998)

Mutagen

in vitro construct

(Winberg et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-sema-II.K}

Cytology

Description

UAS regulatory sequences drive expression of Sema2a.

(Winberg et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Sema2a

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^how-24B

(Ayoob et al., 2006)

Phenotype Manifest In

larval transverse nerve, with Scer\GAL4^A51

(Winberg et al., 1998)

larval transverse nerve, with Scer\GAL4^how-24B

(Ayoob et al., 2006, Winberg et al., 1998)

larval VL3/4 motor neuron, with Scer\GAL4^how-24B

(Ayoob et al., 2006)

Detailed Description

Statement

Reference

Expression of Sema2a^UAS.cKa under the control of Scer\GAL4^GMR83H12 leads to subtle changes in P-Fr neurons process targeting when compared to control adults.

(Xie et al., 2019)

Expression of Sema-2a^Scer\UAS.cKa in all neurons (under the control of Scer\GAL4^elav.PLu), epidermis (Scer\GAL4^wg-Gal4), trachea (Scer\GAL4^tracheal) and oenocytes (Scer\GAL4^salm-459.2) does not alter sensory axon projections or result in axon stalling.

(Bates and Whitington, 2007)

Expression of Sema-2a^Scer\UAS.cKa in all muscles, with the Scer\GAL4^how-24B driver, leads to aberrant formation of the transverse nerve in 27.9% of cases, and inhibits the innervation of muscles 6 and 7 by the RP3 motoneuron, causing axons to stall or enter inappropriate areas.

(Ayoob et al., 2006)

Scer\GAL4^how-24B-mediated expression causes failure of the transverse nerve to form. Scer\GAL4^A51-mediated expression specifically repels the lateral branch of SNa.

(Winberg et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^how-24B, Sema2a^UAS.cKa has abnormal neuroanatomy phenotype, suppressible by PlexB^KG00878

(Ayoob et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^how-24B, Sema2a^UAS.cKa has larval transverse nerve phenotype, suppressible by PlexB^KG00878

(Ayoob et al., 2006)

Scer\GAL4^how-24B, Sema2a^UAS.cKa has larval transverse nerve phenotype, suppressible by NetA^UAS.cHa, Scer\GAL4^how-24B

(Winberg et al., 1998)

Suppressor of

Statement

Reference

Sema2a^UAS.cKa, Scer\GAL4^how-24B is a suppressor of larval transverse nerve phenotype of NetA^UAS.cHa, Scer\GAL4^how-24B

(Winberg et al., 1998)

Sema2a^UAS.cKa, Scer\GAL4^l(3)H94-H94 is a suppressor of larval transverse nerve phenotype of Fas2^UAS.cLb, Scer\GAL4^l(3)H94-H94

(Winberg et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

A plexB^KG00878 background greatly reduces the tranverse nerve phenotype found in Sema-2a^Scer\UAS.cKa; Scer\GAL4^how-24B mutants (from aberrant formation in 27.9% to 12.2% of hemisegments).

(Ayoob et al., 2006)

Scer\GAL4^how-24B-mediated coexpression of NetA^Scer\UAS.cHa and Sema-2a^Scer\UAS.cKa allows correct innervation by the transverse nerve and the RP3.

(Winberg et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Sema2a^UAS.cKa/Scer\GAL4^salm-459.2 rescues Sema2a⁰³⁴⁷⁴

(Bates and Whitington, 2007)

Comments

Expression of Sema-2a^Scer\UAS.cKa under the control of Scer\GAL4^peb-GAL4 is sufficient to rescue the ventromedial targeting defects of Mmus\Cd8a^Mz19-positive projection neuron dendrites seen in Sema-2a⁰³⁴⁷⁴ Sema-2b^unspecified double homozygous flies.

(Sweeney et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Sema-2a^Scer\UAS.cKa

Sema2a^Scer\UAS.cKa

Sema2a^UAS.cKa

sema-II^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kolodkin

(Winberg et al., 1998)

Secondary FlyBase IDs

References (6)

Report Sections

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